BIOCHEMICAL SOCIETY TRANSACTIONS

VOL . 27 , NO . 1 1999

667th Leicester Meeting

A1 Trimeric G-proteins: structure, mechanism and regulation
P.B. Sigler 1

A2 Time-resolved crystallography of protein intermediates
K. Moffat 1

A3 Protein kinase structure and mechanism
L. Johnson 1

A4 Single molecule enzyme kinetics: application to myosin ATPases
C.R. Bagshaw 2

A5 The structure of bovine mitochondrial F1ATPase: an example of rotary catalysis
A.G.W. Leslie 2

A6 Time-resolved intermediate trapping and X-ray crystallographic studies of enzyme
mechanism: isocitrate dehydrogenase and nuclease catalysts
B.L. Stoddard 2

A7 Galactose and amine oxidases: making the most of tyrosine residues
S. Phillips 2

A8 Antibiotics and CoA enzymology: some useful connections
B. Shaw 3

A9 The enzymology of polyketide antibiotic biosynthesis
P.F. Leadlay 3

A 10 DNA gyrase as a drug target
A. Maxwell 3

A11 Dihydropteroate synthase: an old drug target revisited
J.P, Derrick 3

A12 Penicillin and cephalosporin biosynthesis
J. Hajdu 4

A13 Mechanistic diversity of p-lactamases
J.-M. Frere 4

A 14 Protein antibiotics and their inhibition
C. Kleanthous 4

B I The maternal, fetal and postnatal somatotrophic axes in intra-uterine growth retardation
P. Gluckman 4

B2 Control of fetal growth by genomic imprinting
W. Reik 5

B3 Perinatal hormone levels and their role in normal/abnormal development and function
of the male reproductive system 5
R. Sharpe

B4 Gender-specific brain formation of oestrogen in behavioural development 5
J.B. Hutchinson

B5 Glucocorticoids and fetal programming 5
J.R. Seckl

B6 Genetic dissection of glucocorticoid receptor function 6
H. Reichardt

B7 Early environmental events regulate neuroendocrine development 6
M. Meaney

B8 Deiodinase protection of the fetus from thyroid hormones 6
D.L. St Germain

B9 Intra-uterine programming of hypertension: the role of the renin-angiotensin system 6
S.C. Langley-Evans

BIO Neuroendocrine adaptations of the fetus to nutrient restriction 7
LC. McMillen

B 11 Programming of hepatic and peripheral tissue insulin sensitivity by maternal protein 7
restriction
S.E. Ozanne

B12 Maternal nutrition and endocrine programming of fetal adipose tissue development 7
M.E. Symonds

BIS Pre-adrenarche androgens and glucocorticoids and blood pressure control 7
J. Honour

B14 Fetal programming of adult diseases 8
D. Barker
Cl Overview of the biochemistry curriculum
E.J. Wood 8

C2 Core biochemistry: the Society's view
J.M. Wrigglesworth 8

C3 Core biochemistry: a European perspective
J. Aghion 8

C4 Core biochemistry: lessons from biological sciences
L. Jervis 9

C5 Core biochemistry: the necessity for chemistry
R.J.H. Davies 9

C6 Core biochemistry: an industrial perspective
D. Boxer 9

C7 Core biochemistry: a pharmaceutical industry perspective
M.J.B. Brown 9

Dl Properties of the hyaluronan synthase from Group A Streptococcus pyogenes
P.H. Weigel 10

D2 Mammalian hyaluronan synthases: investigation of functional relationships in vivo
A.P. Spicer 10

D3 Mammalian hyaluronan synthases and their functions
N. Itano 10

D4 Colon-derived smooth-muscle cells treated with double-stranded RNA (poly 1:C)
increase adherence of mononuclear leucocytes via hyaluronan-CD44 interactions
V.C. Hascall 10

D5 Structural regulation of hyaluronan binding to proteins
A.J. Day 11

D6 The conformations of hyaluronan in aqueous solution: comparison of theory and
experiment
J. Sheehan 11

D7 New approaches to the investigation of hyaluronan networks
T. Hardingham 11

D8 Biomedical applications of hyaluronan
E. Balazs 11
D9 Hyaluronan in embryogenesis
C. Archer 12

DIO Hyaluronan injoint cavitation
A.A. Pitsillides 12

Dll Hyaluronan receptors: the regulation of the cytoskeleton and their impact on skin
wound
E. Turley 12

D12 The chondrocyte pericellular matrix: a model for hyaluronan-mediated cell-matrix
interactions
C.B. Knudson 12

D13 TSG-6: a hyaladherin associated with inflammation
H.-G. Wisniewski 13

D14 Mode of action of hyaluronate enhancement of haemopoiesis
H. Ponta 13

El Introduction: importance of oestrogen, xenoestrogen and phytoestrogen metabolism in
breast cancer risk?
H. Wiseman 13

E2 Oestrogen and phytoestrogen metabolism: role of the gut microflora
1. Rowland 13

E3 Phytoestrogens in human milk and other biomatrices
A.A. Franke 14

E4 Phytoestrogen sulphoconjugates as inhibitors of sterol sulphatase
W.M. Keung 14

E5 4-Hydroxylation of oestrogens as a marker of breast cancer risk
J.G. Liehr 14

E6 Aromatase and breast cancer risk
M. Dowsett 14

E7 Regulation ofsteroid sulphatase and oestradiol 173-hydroxysteroid dehydrogenase in
breast cancer
M.J. Reed 15

Fl Metabolic pathway engineering of plant secondary metabolites
A.J. van Tunen

F2 Genetic engineering of plant secondary metabolism using particle bombardment
M.J. Leech 15

p3 Micromanipulation measurement of plant cell mechanical properties
C.R. Thomas 15

p4 Competitive production systems: cost/benefit/scale effects
P. Lapinskas 16

F5 Ascorbate: the light in mid-pathway
N. Smirnoff 16

F6 Special effects from plants
D.W.M. Smiley 16

F7 Flavonoids: antioxidants in vino veritas or the cup that cheers?
A. Crozier 16

F8 Manipulating the glucosinolate content of Brassicas
R. Mithen 17

F9 Fatty acid oxidation; tasteful tunes played on the green notes
R. Casey 17

FIO The terpenoid pathway: closing the loop
M.H. Beale 17

Fl I Genetic engineering of the plant isoprenoid pathway: all things considered?
T.J. Bach 17

F12 Checks and balances in the lipid pathways/network
T. Slabas 18

F13 Turnover and sequestration of plant secondary products
R. Edwards 18

F14 Forging the link; international networking
I.D.G. Bartle 18

Gl Live control of the living cell
H.V. Westerhoff 18

G2 Design of gene circuitry by natural selection: analysis of the lactose catabolic system in
Escherichia coli
M.A. Savageau 19

G3 Theoretical studies on how ATP supply meets ATP consumption
B. Korzeniewski 19

G4 New insights into metabolic pathway optimization by analogy with industrial
manufacturing processes
M.Cascante 19

G5 Enzyme kinetics from a metabolic perspective
A. Cornish-Bowden 19

G6 Modelling lipid metabolism in plants: a slippery problem?
J.L. Harwood 20

G7 Modelling metabolism in vivo: approaches using NMR
R.A. lies 20

G8 Control and structural design of glycolysis: an evolutionary approach
R. Heinrich 20

G9 Traditional concepts of metabolic control mislead more than enlighten
D.A. Fell 20

GIO Using practicals to teach metabolic control analysis (MCA): a worked example
P. Quant 21

Gil Towards understanding the extras of metabolic pathways: the implementation of
quantitative analyses
J.L. Snoep 21

G12 Kinetics for the numerically challenged
K.F. Tipton 21

G13 Using computers to learn about metabolism
P. Mendes 21

G14 Mrs. Spratt, young penguins and drunken elephants: teaching metabolic regulation in
relation to health and disease requires a whole-body approach
M. Watford 22

G15 Applying the learning: the case for teaching in industry
D. Myles 22

G16 Overview: linking research and teaching
M. Brand 22

HI Shedding of the interleukin-6 receptor: mechanisms and physiological consequences
S. Rose-John 22

H2 Characterization of the tumour-necrosis-factor (TNF (alpha)-converting enzyme
proteins
D.R Cerretti 23

H3 Interleukin-6 receptor shedding: a role for members of the ADAM (a disintegrin and I
metalloproteinase-like) family
P.I. Croucher 21

H4 Angiotensin-converting enzyme and the amyloid precursor protein secretases
N.M. Hooper 23

H5 Proteolytic processing of (beta)-amyloid precursor protein and presenilins
H. Steiner

mechanismss controllins the shedding of transmembrane molecules
J.arribas 24

activated receptors: novel functions for serine proteases
MicU 24

ADAMs (a disintegrin and metalloproteinase-like) proteinases as membrane
protoin secratases
A.J.TUNER 24

J4 Multichannel affinity sensors for environmental monitoring
D.C. Cullen 27

J5 Biacore: an affinity biosensor for characterization of biomolecular interactions
M. Malmqvist 28

J6 Cholera toxin and GM1:a model membrane study of lAsy
C.H. Maule 28

J7 Molecular imprinted biosensors
K. Mosbach 28

J8 Holographic biosensors
C.R. Lowe 28

Kl Flavocytochromes: structures and implications for electron transfer
F.S. Mathews 29

K2 Flavocytochromes: transceivers and relays in biological electron transfer
S.K. Chapman 29

K3 Structural studies of the flavoprotein reductase component of the P-450 mono-
oxygenase system
G.C.K. Roberts 29

K4 Flavocytochrome P-450 BM3: electron transfer and its control in a model P-450
A.W.Munro 29

K5 Electron transfer in trimethylamine dehydrogenase and electron-transferring
flavoprotein
N.S.Scrutton 30

K6 Methylamine dehydrogenase: structure and function of electron-transfer complexes
V.L. Davidson 30

K7 Ribonucleotide-reductase-coupled electron-/proton-transfer mechanisms
B.M. Sjoberg

K8 Pyrroloquinoline quinone (PQQ)-dependent methanoi dehydrogenase and glucose .
dehydrogenase
C. Anthony 31

______________________________________________




E5 Phosphoinositide 3-kinase knock-out mice: role of p85oc in B cell development and
proliferation
A. Fruman 73


E6 The role of phosphoinositide 3-kinase in the regulation of cell survival and apoptosis
J. Downward 74


E7 Phosphoinositide 3-kinase signalling: no lipids
M.P. Wymann 74

E8 Mechanisms of COPI-vesicle biogenesis
F. Wieland 74


E9 Lipid-protein interactions in the biogenesis of neurosecretory vesicles
W.B. Huttner 74


E 10 Intracellular transport and organelle morphology in cell lines with inducible
overexpression ofphospholipase D I (PLDI) or of a PLD I-derived antisense fragment
N. Ktistakis 75


E11 The small GTPase ADP-ribosylation factor (ARF) regulates phosphoinositide 4-kinase
P in the Golgi complex
M.A. De Matteis 75


E 12 ADP-ribosylation factor (ARF), a multi-functional GTPase as a co-ordinator of
membrane traffic: is ARF-regulated phospholipase D the answer to everything?
S. Cockcroft 75


E 13 Phosphoinositides and neurotransmitter release
G. Schiavo 75


E14 Phospholipase C-§ and related molecules
H. Yagisawa 76


E 15 Localization and regulation of type II PIPkins (Ptdlns5P 4-kinases)
R.F. Irvine 76


E 16 Regulation of early endosome dynamics by early endosomal antigen-1 (EEAI): a
phosphatidylinositol 3-phosphate-binding protein
M.J. Clague 76




E 17 Regulation of endocytic membrane traffic by phosphatidylinositol 3-phosphate
H. Stenmark 76



E 18 Lipid kinases and trans-Golgi network membrane dynamics
S.Ponnambalam 77


E 19 Phosphatidylinositol 3,5-bisphosphate: a novel inositol lipid linking stress responses to
membrane trafficking in yeast
S.K. Dove 77


E20 Analysis of protein and vesicle trafficking using green fluorescent protein
J.M. Tavare 77


E21 Role for ADP-ribosylation factors and phosphoinositides in insulin-regulated
membrane trafficking in 3T3-LI adipocytes
G.W. Gould 77


E22 Phosphoinositide 3-kinase effector molecules
P.J. Cullen 78
_______________________________________________


BIOCHEMICAL SOCIETY TRANSACTIONS
VOL. 27, NO. 2, 1999


Single molecule enzyme kinetics: applications to C. R. BAGSHAW AND P. B. CONIBEAR 33
myosin ATPases

The structure of bovine mitochondrial Fi-ATPase: an A. G. W. LESLIE, J. P. ABRAHAMS, K. BRAIG, R. LUTTER, 37
example of rotary catalysis R. I MENZ, G. L. ORRISS, M. J. VAN RAAIJ AND
J. E. WALKER


Visualizing enzyme intermediates using fast B. L. STODDARD 42
diffraction and reaction trapping methods: isocitrate
dehydrogenase

DNA gyrase as a drug target A. MAXWELL 48

Dehydropteroate synthase: an old drug revisited H. G. VINNICOMBE AND J. P. DERRICK 53


Mechanistic diversity of beta -lactamases J.-M. FRERE, A. DUBUS, M. GALLENI, A. MATAGNE AND 58
G.AMICOSANTE


Protein antibiotics and their inhibitors G. KLEANTHOUS, R. JAMES, A. M. HEMMINGS AND 63
G. R. MOORE


colloquium: Endocrine Control of Perinatal Programming in Health and
Disease

The maternal, fetal and postnatal somatographic M. H. OLIVER, F. H. BLOOMFIELD, J. E. HARDING, 69
axes in intrauterine growth retardation B. H. BREIER, N. S. BASSETT AND P. D. GLUCKMAN


Glucocorticoids and fetal programming J. R. SECKL, M. J. NYIRENDA, B. R. WALKER AND 74
K. E. CHAPMAN


The Cre/loxP system - a versatile tool to study H. M. REICHARDT. C. KELLENDONK, F. TRONCHE AND 78
glucocorticoid signalling in mice G. SCHÜTZ


Development effects of thyroid hormone: the role of D. L. ST. GERMAIN 83
deiodinases m regulatory control

Intrauterine programming of hypertension: the role S. C. LaNGLEY-EVANS, R. C. SHERMAN, - 88
of the renin-angiotensin system S. J. M. WELHAM, M. 0. NWAGWU, D. S. GARDNER
AND A. A. JACKSON


Programming of hepatic and peripheral tissue insulin S. E. OZANNE 94
sensitivity by maternal protein restriction

Maternal nutrition and endocrine programming of M. E. SYMONDS AND T. STEPHENSON 97
fetal adipose tissue development

colloquium: The Biology of Hyaluronan

Properties of the hyaluronan synthase from Group A V. L. TLaPAK-SIMMONS, C. HELDERMON, 105
Streptococcus pyogenes E. S. KEMPNER AND P. H. WEIGEL

Mammalian hyaluronan synthases: investigation of A. P. SPICER AND T. K. NGUYEN 109
functional relationships in vivo

The structure and regulation of hyaluronan-binding A. J. DAY 1 15
proteins

The conformations of hyaluronan in aqueous J. SHEEHAN, A. BRASS AND A. ALMOND 121
solution: comparison of theory and experiment

New approaches to the investigation of hyaluronan T. HARDINGHAM, B. C. HENG AND P. GRIBBON 124
networks

Hyaluronan in Joint cavitation A. C. WARD, G. P. DOWTHWAITE AND 128
A. A. PITSILLIDES

Receptor for hyaluronan-mediated motility W.-F. CHEUNG, T. F. CRUZ AND E. A. TURLEY 135
(RHAMM), a hyaladherin that regulates cell
responses to growth factors

The chondrocyte pericellular matrix: a model for . C. B. KNUDSON, G. A. NOFAL, L. PAMINTUAN AND 142
hyaluronan-mediated cell-matrix interactions D. J. AGUIAR

colloquium: G-Protein-Coupled Receptor Signalling in the CentralNervous System


Diversity in the signalling and regulation of G. MILUGAN, D. A. GROARKE, A. MCLEAN, R. WARD, 149
G-protein-coupled receptors C. W. FONG, A. CAVALLI AND T. DRMOTA


Regulatory mechanisms of alfa lb iB-adrenergic receptor S. GOTECCHIA AND S. MHAOUTY-KODJA 154
function

Promiscuity and fidelity in receptor-G-protein F. KALKBRENNER, A. ABEL, N. WITTAU AND 158
coupling: cell cycle-dependent coupling of the G. SCHULTZ
vasopressin Vi receptor

Heterologous mammalian expression systems for E. HERMANS, S. R. NAHORSKI AND R. A. J. GHALUSS 164
investigating the properties of metabotropic
glutamate receptors


Evidence that a novel metabotropic glutamate Z. A. BORTOLOTTO AND G. L. COLUNGRIDGE 170
receptor mediates the induction of long-term
potentiation at CA I synapses in the hippocampus

Mechanisms of action of anti-psychotic drugs P. G. STRANGE 175

colloquium: Enzyme-Catalysed Electron/Radical Transfer

Flavocytochromes: structures and implications for L. M. CUNANE. Z.-W. CHEN, R. C. E. DURLEY, 179
electron transfer J. D. BARTON AND F. S. MATHEWS

Flavocytochromes: transceivers and relays in S. K. CHAPMAN, F. WELSH, K MOYSEY, C. MOWAT, 185
biological electron transfer M. K. DOHERTY, K. L. TURNER, A. W. MUNRO AND
G. A. REID

Flavocytochrome P-^O BM3: a paradigm for the A. W. MUNRO, M. A. NOBLE, C. S. MILES, 190
analysis of electron transfer and its control in the S. N. DAFF, A. J. GREEN, L. QUARONI, S. RIVERS,
p-450s T. W. B. OST, G. A. REID AND S. K. CHAPMAN

Electron transfer in trimethylamine dehydrogenase N. S. SCRUTTON, J. BASRAN, E. K. WILSON, 196
and electron-transferring flavoprotein K. K. CHOHAN, M.-H. JANG, M. J. SUTCLIFFE AND
R. HILLE

Methylamine dehydrogenase: structure and function V. L. DAVIDSON 201
of electron transfer complexes

Electron transfer and coupled processes in protein F. A. ARMSTRONG 206
film voltammetry

colloquium: Membrane Protein Secretases

Generation and function of the soluble interleukin-6 J. MÜLLBERG, P. VOLLMER, K. ALTHOFF, P. MÄRZ AN D 211
receptor S. ROSE-JOHN

Characterization of the tumour necrosis factor D. P. CERRETTI 219
converting enzyme, TACE/ADAM 17

lntcrteukin-6 receptor shedding: a posrofor P. I. CROUCHER, F. WANG AND P. G. HARGREAVES 224
members of the ADAM family

Angiotensin-converting enzyme and the amyloid N. M. HOOPER, S. PARVATHY. E. H. KARRAN AND 229
P^cursor protein secretases A.J.TURNER

^oteolytic processing and degradation of H. STEINER, A. CAPELL AND C. HAASS 234
Mfhuiner^ disease relevant proteins

mechanisms controlling the shedding of A. MERLOS-SUÄREZ AND J. ARRIBAS 243
transmembrane molecules


Proteinas-activated receptors: a growing family of O.DERY AND N. W. BUNNETT 246
heptahelical receptors for thrombin, trypsin and
tryptase

Role for ADAM -family proteinases as membrane A. J. TURNER AND N. M. HOOPER 255



Live control of the living cell W. C. VAN HEESWIJK, B. M. BAKKER, B. TEUSINK, 261
B. N. KHOLODENKO, 0. J. G. SOMSEN, J. L. SNOEP
AND H. V. WESTERHOFF


Design of gene circuitry by natural selection: analysis M.A.SAVAGEAU 264
of the lactose catabolic system in Escherichio coli

Theoretical studies on how ATP supply meets ATP B. KORZENIEWSKI 271
demand

New insights into metabolic pathway optimization F. ORTEGA, E. MARTI AND M. CASCANTE 276
by analogy with industrial manufacturing processes

Enzyme kinetics from a metabolic perspective A. CORNISH-BOWDEN 281

Modelling lipid metabolism in plants: a slippery J. L. HARWOOD, U. S. RAMU, R. A. PAGE AND 285
problem? P. A. QUANT

Modelling metabolism in vivo: approaches using NMR R. A. ILES, J. S. BEECH, S. P. BURNS AND R. D. COHEN 289

The structural design ofglycolysis: an evolutionary R. HEINRICH. E. MELENDEZ-HEVIA, F. MONTERO, 294
approach J. C. NUNO, A. STEPHANI AND T. G. WADDELL

colloquium: Oestrogen Metabolism and Breast Cancer Risk

Importance of oestrogen,xenoestrogen and H.WISEMAN 299
phytoestrogen metabolism in breast cancer risk

Metabolism ofoestrogens and phytoestrogens: role I. ROWLAND, H. WISEMAN, T. SANDERS, 304
of the gut microflora H. ADLERCREUTZ AND E. BOWEY

Phytoestrogens in human biomatrices including A. A. FRANKE, M. C. Yu, G. MASKARINEC, P. FANTI, 308
breast milk W. ZHENG AND L. J. CUSTER

4-Hydroxylation of oestrogens as a marker for J. G. LIEHR 318
mammary tumours

Regulation of steroid sulphatase and oestradiol A. PUROHIT, A. SINGH AND M. J. REED 323
17 beta -hydroxysteroid dehydrogenase in breast cancer

colloquium: Biosensors: Into the 21st Century

Analysis of structure-activity relationships with M. H. V. VAN REGENMORTEL 329
biosensors

Biosensors: past, present and future H.A. 0. HILL AND J.J.DAVIS 331

BIACORE: an affinity biosensor system, for M. MALMQVIST 335
characterization of biomolecular interactions

Cholera toxin and G (MI ) a model membrane study N. ATHANASSOPOULOU, R. J. DAVIES, P. R. EDWARDS, 340
with lAsys D. YEUNG AND C. H. MAULE

Molecularly imprinted polymers in chemical and K. HAUPT AND K. MOSBACH 344
biological sensing

LI From genomics to aetiology in the multifactorial disease type-1 diabetes
J.A. Todd 1

AI Trimeric G-proteins: structure, mechanism and regulation
P.B. Sigler 1

A2 Time-resolved crystallography of protein intermediates
K. Moffat 1

A3 Protein kinase structure and mechanism
L. Johnson 1

A4 Single molecule enzyme kinetics: application to myosin ATPases
C.R. Bagshaw 2

A5 The structure of bovine mitochondrial F1ATPase: an example of rotary catalysis
A.G.W. Leslie 2

A6 Time-resolved intermediate trapping and X-ray crystallographic studies of enzyme
mechanism: isocitrate dehydrogenase and nuclease catalysts
B.L. Stoddard 2

A7 Galactose and amine oxidases: making the most of tyrosine residues
S. Phillips 2

A8 Antibiotics and CoA enzymology: some useful connections
B. Shaw 3

A9 The enzymology of polyketide antibiotic biosynthesis
P.F. Leadlay 3

A 10 DNA gyrase as a drug target
A. Maxwell 3

All Dihydropteroate synthase: an old drug target revisited
J.P. Derrick 3

A 12 Penicillin and cephalosporin biosynthesis
J. Hajdu 4

Al 3 Mechanistic diversity of p-lactamases 4
J.-M. Frère

A 14 Protein antibiotics and their inhibition 4
C. Kleanthous

B I The maternal, fetal and postnatal somatotrophic axes in intra-uterine growth retardation 4
P. Gluckman

B2 Control of fetal growth by genomic imprinting 5
W. Reik

B3 Perinatal hormone levels and their role in normal/abnormal development and function 5
of the male reproductive system
R. Sharpe

B4 Gender-specific brain formation of oestrogen in behavioural development 5
J.B. Hutchinson

B5 Glucocorticoids and fetal programming 5
J.R. Seckl

B6 Genetic dissection of glucocorticoid receptor function 5
H. Reichardt

B7 Early environmental events regulate neuroendocrine development 6
M. Meaney

B8 Deiodinase protection of the fetus from thyroid hormones 6
D.L. St Germain

B9 Intra-uterine programming of hypertension: the role of the renin-angiotensin system 6
S.C. Langley-Evans

BIO Neuroendocrine adaptations of the fetus to nutrient restriction 7
LC. McMillen

B 11 Programming of hepatic and peripheral tissue insulin sensitivity by maternal protein 7
restriction
S.E. Ozanne

B12 Maternal nutrition and endocrine programming of fetal adipose tissue development 7
M.E. Symonds

B13 Pre-adrenarcheandrogens and glucocorticoids and blood pressure control 7
J. Honour

B14 Fetal programming of adult diseases 8
D. Barker

Cl Overview of the biochemistry curriculum
E.J. Wood 8

C2 Core biochemistry: the Society's view
J.M. Wrigglesworth 8

C3 Core biochemistry: a European perspective
J. Aghion 8

C4 Core biochemistry: lessons from biological sciences
L. Jervis 9

C5 Core biochemistry: the necessity for chemistry
R.J.H. Davies 9

C6 Core biochemistry: an industrial perspective
D. Boxer 9

C7 Core biochemistry: a pharmaceutical industry perspective
M.J.B. Brown 9

Dl Properties of the hyaluronan synthase from Group A Streptococcus pyogenes
P.H. Weigel 10

D2 Mammalian hyaluronan synthases: investigation of functional relationships in vivo
A.P. Spicer 10

D3 Mammalian hyaluronan synthases and their functions
N. Itano 10

D4 Colon-derived smooth-muscle cells treated with double-stranded RNA (poly 1:C)
increase adherence of mononuclear leucocytes via hyaluronan-CD44 interactions
V.C. Hascall 10

D5 Structural regulation of hyaluronan binding to proteins
A.J. Day 11

D6 The conformations of hyaluronan in aqueous solution: comparison of theory and
experiment
J. Sheehan 11

D7 New approaches to the investigation of hyaluronan networks
T. Hardingham 11

D8 Biomedical applications of hyaluronan
E. Balazs 11

D9 Hyaluronan in embryogenesis
C. Archer 12

DIO Hyaluronan injoint cavitation
A.A. Pitsillides 12

DU Hyaluronan receptors: the régulation of the cytoskeleton and their impact on skin
wound
E. Turley 12

D12 The chondrocyte pericellular matrix: a model for hyaluronan-mediated cell-matrix
interactions
C.B. Knudson 12

D13 TSG-6: a hyaladherin associated with inflammation
H.-G. Wisniewski 13

D14 Mode of action othyaluronate enhancement ofhaemopoiesis
H. Ponta 13

El Introduction: importance of oestrogen, xenoestrogen and phytoestrogen metabolism in
breast cancer risk?
H. Wiseman 13

E2 Oestrogen and phytoestrogen metabolism: role of the gut microflora
1. Rowland 13

E3 Phytoestrogens in human milk and other biomatrices
A.A. Franke 14

E4 Phytoestrogen sulphoconjugates as inhibitors of sterol sulphatase
W.M. Keung 14

E5 4-Hydroxylation of oestrogens as a marker of breast cancer risk
J.G. Liehr 14

E6 Aromatase and breast cancer risk
M. Dowsett 14

E7 Regulation ofsteroid sulphatase and oestradiol 173-hydroxysteroid dehydrogenase in
breast cancer
M.J. Reed 15

FI Metabolic pathway engineering of plant secondary metabolites
A.J. van Tunen

F2 Genetic engineering of plant secondary metabolism using particle bombardment
M.J. Leech 15

F3 Micromanipulation measurement of plant cell mechanical properties
C.R. Thomas 15

F4 Competitive production systems: cost/benefit/scale effects
P. Lapinskas 16

F5 Ascorbate: the light in mid-pathway
N. Smirnoff 16

F6 Special effects from plants
D.W.M. Smiley 16

F7 Flavonoids: antioxidants in vino veritas or the cup that cheers?
A. Crozier 16

F8 Manipulating the glucosinolate contentof Brassicas
R. Mithen 17

F9 Fatty acid oxidation; tasteful tunes played on the green notes
R. Casey 17

F10 The terpenoid pathway: closing theloop
M.H. Beale 17

F11 Genetic engineering of the plant isoprenoid pathway: all things considered?
T.J. Bach 17

F12 Checks and balances in the lipid pathways/network
T. Slabas 18

F 13 Turnover and sequestration of plant secondary products
R. Edwards 18

F14 Forging the link; international networking
I.D.G. Bartle 18

G1 Live control of the living cell
H.V. Westerhoff 18

G2 Design of gene circuitry by natural selection: analysis of the lactose catabolic system in
Esch erichia coli
M.A. Savageau 19

G3 Theoretical studies on how ATP supply meets ATP consumption
B. Korzeniewski 19

G4 New insights into metabolic pathway optimization by analogy with industrial
manufacturing processes
M.Cascante 19
G5 Enzyme kinetics from a metabolic perspective
A. Cornish-Bowden 19

G6 Modelling lipid metabolism in plants: a slippery problem?
J.L. Harwood 20

G7 Modelling metabolism in vivo: approaches using NMR
R.A. lies 20

G9 Traditional concepts of metabolic control mislead more than enlighten
D.A. Fell 20

G10 Using practicals to teach metabolic control analysis (MCA): a worked example
P. Quant 21

G11 Towards understanding the extras of metabolic pathways: the implementation of
quantitative analyses
J.L. Snoep 21

G 12 Kinetics for the numerically challenged
K.F.Tipton 21

G 13 Using computers to learn about metabolism
P. Mendes 21

G 14 Mrs. Spratt, young penguins and drunken elephants: teaching metabolic regulation in I
relation to health and disease requires a whole-body approach
M. Watford 22

G 15 Applying the learning: the case for teaching in industry
D. Myles 22

G 16 Overview: linking research and teaching
M. Brand 22

H1 Shedding of the interleukin-6 receptor: mechanisms and physiological consequences
S. Rose-John 22

H2 Characterization of the tumour-necrosis-factor (TNFcx)-converting enzyme
[(TACE)/ADAM17]-deficient mouse and its role in the shedding of cell-surface
proteins
D.P. Cerretti 23

H3 Interleukin-6 receptor shedding: a role for members of the ADAM (a disintegrin and
metalloproteinase-like) family
P.I. Croucher 23

H4 Angiotensin-converting enzyme and the amyloid precursor protein secretases
N.M. Hooper 23

H5 Proteolytic processing of (beta)-amyloid precursor protein and presenilins
H. Steiner 23

H6 Mechanisms controlling the shedding oftransmeinbrane molecules
J. Arribas 24

H7 Protease-activated receptors: novel functions for serine proteases
N.W. Bunnett 24

H8 A role for ADAMs (a disintegrin and metalloproteinase-like) proteinases as membrane
protein secretases
A.J. Turner 24

11 Diversity in the signalling and regulation of G-protein-coupled receptors
G. Milligan 24

12 Metabotropic glutamate receptor signalling
E. Hermans 25

13 Acute and chronic regulation by G-protein-coupled receptors
S. Cotecchia 25

14 Involvement of the N-terminus of neuronal calcium channels in G-protein modulation
A.C. Dolphin 25

15 Cell-cycle-dependent coupling of vasopressin V1-receptor to G q/11 and Gi3
F. Kalkbrenner 25

16 Organization of G-protein-coupled receptors and ion channels at the synapse
P. Somogyi 26

17 Role of G-protein-coupled receptors in adaptative neuronal processes
G.L. Collingridge 26

18 Clinical exploitation of 5-hydroxytryptamine receptor diversity
D. Hoyer 26

19 Dopamine receptor antagonists or inverse agonists as anti-psychotics?
P.G. Strange 26

Jl Analysis of structure-activity relationships with biosensors
M.H.V. Van Regenmortal 27

J2 Design of molecular biosensor surfaces for screening ligand-receptor interactions by
functional assay
H. Vogel 27

J3 Biosensors: past, present and future
H.A.O. Hill 27

J4 Multichannel affinity sensors for environmental monitoring
D.C. Cullen 27

J5 Biacore: an affinity biosensor for characterization of biomolecular interactions
M. Malmqvist 28

J6 Cholera toxin and G M1: a model membrane study of lAsys
C.H. Maule 28

J7 Molecular imprinted biosensors
K. Mosbach 28

J8 Holographic biosensors
C.R. Lowe 28

Kl Flavocytochromes: structures and implications for electron transfer
F.S. Mathews 29

K2 Flavocytochromes: transceivers and relays in biological electron transfer
S.K. Chapman 29

K3 Structural studies of the flavoprotein reductase component of the P-450 mono-
oxygenase system
G.C.K. Roberts 29

K4 Flavocytochrome P-450 BM3: electron transfer and its control in a model P-450
A.W.Munro 29

K5 Electron transfer in trimethylamine dehydrogenase and electron-transferring
flavoprotein
N.S.Scnitton 30

K6 Methylamine dehydrogenase: structure and function of electron-transfer complexes
V.L. Davidson 30

K7 Ribonucleotide-reductase-coupled electron-/proton-transfer mechanisms
B.M. Sjoberg 30

K8 Pyrroloquinoline quinone (PQQ)-dependent methanoi dehydrogenase and glucose
dehydrogenase
C* Anthony 30

K9 Galactose oxidase and topaquinone (TPQ)-dependent amine oxidase
P. Knowles 31

KIO Electron transfer and coupled processes in protein film voltammetry
F.A. Armstrong 31
1 Problem-based learning: scaling-up issues
L. Jervis 31

2 The effect of site-directed mutagenesis of two transmembrane serine residues on
agonist-specific coupling of a cloned human (alpha)2A-adrenergic receptor
J.E. Rudling and P.D. Evans 31

3 Muscarinic receptor (M^-evoked increase in [Ca2+] i in SH _SY5Y cells inhibits
noradrenaline release
D.J. Roberts, C. Peers and P.F.T. Vaughan 32

4 Characterization of purinoceptor-mediated Ca2+ oscillations in L-fibroblasts
R.J. Davis, R.A.J. Challiss and S.R. Nahorski 32

5 Cultured spinal cord cells take up and release pH]noradrenaline: evidence for intrinsic
noradrenergic neurons
J.R. Purkiss, L.M. Fris, S. Doward and C.P. Quinn 32

6 How does the internalization of G-protein-coupled receptor agonists relate to the
intemalization of their receptors?
J.A. Koenig, R. Kaur and P.P.A. Humphrey 32

7 Distribution of histamine H^-receptor binding in the normal human basal ganglia:
comparison with Huntington's disease and Parkinson's disease cases
R.E. Goodchild, J.A. Court, LHobson, M.A. Piggott, R.H. Perry, P. Ince, E. Jaros
and E.K. Perry 33

8 Molecular determinants for high-affinity binding to the vasopressin V1a receptor
S.R. Hawtin and M. Wheatley 33

9 Naloxone benzoylhydrazone inhibits K+-evoked glutamate release from rat brain slices
B. Nicol, D.J. Rowbotham, D. Smart, A.T. McKnight and D.G. Lambert 33

10 Effects ofsteroidal neuromuscular-blocking drugs on pH]noradrenaline release from
SH-SY5Y cells
T.M. Cembala, B.L. Appadu and D.G. Lambert 33

11 Gonadotropin-releasing hormone receptors with added cytoplasmic C-terminal tails
undergo accelerated (beta)-arrestin-dependent internalization
A. Heding, M. Vreci, A. Hanyaloglu, P.L. Taylor, R. Sellar, B. Byrne, G.B. Willars
and K.A. Eidne 34

12 A comparative study of growth factor receptor and G-protein-coupled receptor
phosphoinositide and Ca2+ signalling in SH-SY5Y neuroblastoma cells
L.M. Wheldon, P.J. White, S.R. Nahorski and G.B. Willars 34
13 Absence of rapid desensitization and agonist-dependent phosphorylation of the
mammalian gonadotropin-releasing hormone receptor is associated with the absence of
a cytoplasmic C-terminal tail
G.B. Willars, S.R. Nahorski, K.A. Eidne and A. Heding 34

14 Phosphorylation and functional regulation of the (beta)2-adrenergic receptor by the
phospholipase-C-coupled M^muscarinic receptor
D.C. Budd, K.W. Young, R.A.J. Challiss and A.B. Tobin 34

115 Calmodulin-dependence ofpresynaptic metabotropic glutamate receptor signalling
V. O'Connor, 0. El Far, E. Bofill-Cardona, C. Nanoff, M. Freissmuth, J.M. Airas,
H. Betz and S. Bohm 35

15 Biochemical characterization of morphological differentiation of malignant epithelial
cells induced by contact with peripheral nerve tissue
G. Cuttle and S.J. Pape 35

16 Screening for CCKA-binding inhibitors: a comparison of combinatorial chemistry
approaches
L. Clark, B. Evans, A. Pipe, S. Arpino and M. Banks 35

17 Studies with compounds that enhance the binding of [125I]-labelled ovine CRF in a
recombinant human CRF-1 receptor scintillation proximity assay
V. Shield, L. Clark, S. and M. Banks 35

18 A single amino acid (Glu-146) governs the substrate specificity of human
catecholamine sulphotransferase SULTIA3
R. Dajani, A. Hood and M. Coughtrie 36

19 Hydrogen bonding and protein pertubation in (beta)-lactam acylenzymes of Streptococcus
pneumoniae penicillin-binding protein PBP2x
A.-S. Wilkinson, C.W. Wharton, R. Chittock, S. Ward, M.G.P. Page and
J.J. Goodall 36

20 Investigation of cytochrome P-450 3A4 by surface-enhanced resonance Raman
scattering
I.D.G. MacDonald and M.P. Pritchard 36

21 Studies ondeacetoxycephalosporinCsynthase
M.D. Lloyd, H.-J. Lee, J.E. Baldwin, C.J. Schofield, J.M. Charnock and C.D. Garner 36

22 Evaluation of a kinetic approach to the determination ofcatalytic-site content in
enzymes and enzyme-like catalysts
S. Sonkaria, S. Gul, M. Resmini and K. Brocklehurst 37

23 Investigation of electrostatic and hydrogen and caricain bonding interactions of
caricain Asp-158—>Asn with time-dependent inhibitors
S. Hussain, K.K. Alien, LF. Connerton, N.J. Cummings, S. Gul, A. Khan,
M.A.J. Taylor, E.W. Thomas, C. Verma and K. Brocklehurst 37

24 Rho-GDI-Rac interaction by NMR
1. Barsukov, L.-Y. Lian, R. Badii, K.-H. Sze and G.C.K. Roberts 37

25 Structural studies of histidines in zinc (beta)-lactamases and their interactions with
inhibitors
C. Damblon, C.L.Y. Lian, R.P. Soto, M.H. Villadares, M. Galleni, J.M. Frère and
G.C.K. Roberts 37

26 Investigation of chymotrypsin-ligand complexes using IR spectroscopy: a model for
the study of (beta)-lactamases?
J.J. Goodall, A.-S. Wilkinson and C.W. Wharton 38

27 Characterization of Dictyostelium myosin II mutated in the converter region
P.A. Kubiman, N. Sasaki, R. Ohkura, K. Sutoh and C.R. Bagshaw 38

28 Kinetic mechanism of aspartate- (beta)-semialdehyde dehydrogenase and its interaction with
small molecule inhibitors
C.J. Greenwood, K.J. Moore, T. Allinson, G. Burton, C. Frydych, F. Harrington,
N. Nicholson, M. Hartley, M. Pearson and A.J. Pope 38

29 Site-directed mutagenesis in the active site of Rhodotorula gracilis D-amino-acid
oxidase
M.S. Pilone, G. Molla, C. Harris, D. Porrini, C. Vegezzi, S. Campaner and L. Pollegioni 38

30 Mechanistic insights into substrate turnover and inhibition ofmetallo-P-lactamases
M.J.B. Brown, M. Gilpin, D. Witty, J. Bateson and A.J. Pope 39

31 The structure, function and convergent evolution of intron-encoded homing
endonucleases
B.L. Stoddard, M. Jurica, P. Heath and K. Flick 39

32 3-Carbonyl-substituted glutathione conjugates as inhibitors of Onchocerca vovulus
glutathione S-transferase 2
P.M. Brophy, A.M. Cambell, A.-M. van Eldick, P.H. Teesdale-Spittle and M.F. Wang 39

114 Structure and function studies ofhydroxymethylbilane synthase using SRS and ESRF
P.F. Faulder, Y.P. Nieh, J. Raftery, J. Habash, A. Haedener, S. McSweeney,
F. Schotte, T. Ursby, M. Wuiff, A.W. Thompson and J.R. Helliwell 39

33 CD spectroscopy in hyaluronan research
G. Siligardi and R. Hussain 40

34 An N-terminal link protein peptide stimulâtes biosynthesis of collagen and
proteoglycans by explants of human articular cartilage
H. Liu, L.A. McKenna and M.F. Dean 40

35 Hyaluronan bound to CD44 on keratinocytes is displaced by hyaluronan
decasaccharides and not hexasaccharides
R. Tammi, D. MacCallum, V.C. Hascall, J.-P. Pienimaki, M. Hyttinen and
M. Tammi 40

36 Epidermal growth factor regulates keratinocyte hyaluronan metabolism
J.-P. Pienimaki, R. Tammi, K. TOrroneii and M. Tammi 40

37 Characterization of trans-synovial flow plateau' caused by high-molecular-mass
hyaluronan
D. Scott, J.R. Levick, P.J. Coleman and R.M. Mason 41

38 A simple theory for concentration polarization during ultrafiltration across a partially
reflecting membrane in a stirred cell
J.R. Levick 41

39 Serum chondroitin sulphate epitopes in rheumatoid arthritis
J. Middleton, S. White, E. Parry, C. Jackson, A. Darby, J. Dixey and B. Ashton 41

40 The expression of mucin genes in Barretts oesophagus
G.S. Arul, N. Myerscough, M. Moorghen, R.D. Spicer, D. Alderson and A.P. Corfield 41

41 Regulation of the cell-surface hyaluronan receptor, CD44
P. Townsend, C. Lewis, J. Legg and C. Isacke 42

42 Recovery of mRNA from chrondocytes in agarose
Z.V. Hazlehurst, V.L. Church, S.M. Kennedy and B.A. Ashton 42

43 Co-localization of a proteoglycan epitope and type X collagen by human intervertebral
disc cells in vitro
S. Roberts, E. Johnson, B. Garguilo, B. Caterson and A. Kwan 42

44 Synthesis of hyaluronan by bacterial fermentation
C.M. Lockett and J.N. Hunter 42

45 Immunolocalization of matrix metalloproteinase-3 (stromelysin-I) in carotid artery
plaques
A.D. Khattab, J. Bowley, I.S. Ali, A. Bowley, R.R. Dils and M.Z. Rana 43

46 Hyaluronic acid fragments activate nuclear factor (NF)KB in ECV304 cells via its
principal cell-surface receptor CD44
K.A. Fitzgerald and L.A.J. CTNeill 43

48 Characterization of a Leu—>Trp mutant of the flavodehydrogenase domain of
flavocytochrome b2
R. Moysey, F. Welsh, S.K. Chapman and G.A. Reid 43

49 Bio 1: is it a cytochrome P-450?
A.J. Green, A.W. Munro, S.L. Rivers, S.K. Chapman and G.A. Reid 44

50 Determinants of substrate binding in flavocytochrome P-450 BM3
M.A. Noble, C.S. Miles, G.A. Reid, S.K. Chapman and A.W. Munro 44

51 Catalytic properties of key active-site mutants of flavocytochrome P-450 BM3
M.A. Noble, C.S. Miles, G.A. Reid, S.K. Chapman and A.W. Munro 44

52 The interaction of nitric oxide with flavocytochrome P-450 BM3
L. Quaroni, W.E. Smith, M.A. Noble and A.W. Munro 44

53 Analysis of flavocytochrome P-450 BM3 using resonance Raman spectroscopy
A.W. Munro, L. Quaroni, I.D.G. MacDonald, W.E. Smith, J. Hudecek,
V. Baumruk and P. Anzenbacher 45

54 Altering the electron acceptor specificity of flavocytochrome b2
F. Welsh, S. Rivers, S.K. Chapman and G.A. Reid 45

55 Voltammetric navigation of a flavocytochrome film
K. Thrner, M. Doherty, S. Chapman, D. Heering and F. Armstrong 45

56 Stepwise electron transfer to 6-5-cysteinyl FMN in trimethylamine dehydrogenase
J. Basran, M.-H. Jang/M.J. Sutcliffe, R. Hille and N.S. Scrutton 45

57 Substrate inhibition in wild-type and mutant trimethylamine dehydrogenases
P. Roberts, J. Basran, M. Mewies, R. Hille and N.S. Scrutton 46

58 Electron transfer in tp-hydroxylation: analysis of rubredoxin reductase and rubredoxin
H.J. Lee, J. Basran, L.-Y. Lian and N.S. Scrutton 46

59 Structure and mechanism of an opiate-transforming redox enzyme: morphinone
reductase
D.H. Craig, N.C. Bruce, P.C.E. Moody and N.S. Scrutton 46

60 Study of intramolecular electron transfer and catalytic action of quinohaemoprotein
alcohol dehydrogenase from Gluconobacter sp. 33
A. Ramanavicius, J. Razurniene, V. Laurinavicius, L. Marcinkeviciene,
1. Bachmatova, R. Meskys and R. Rudomanskis 46

61 Site-directed mutagenesis of an active-site residue in Salmonella typhimurium
dehydroquinate synthase
M.A. Skinner and K.A. Brown 47

62 Construction and expression of the truncated forms of the katG gene from
Mycobacterium tuberculosis
Jesmin, J.M. Nagy, A.E.G. Cass and K.A. Brown 47

63 Expression and preliminary purification of a Zn2+ form of Escherichia coli
dehydroquinate synthase
M. Shafiq, M.A. Skinner and K.A. Brown 47

64 Purification of 5-enolpyruvyJshikirnate-3-phosphate synthase from Haemophilus
influenzae 47
K. A. BROOWN

65 Regulation ofATP synthesis and proton handling in ischaemically exercising skeletal
muscle
G.J. Kemp, M. Roussel, D. Bendahan, Y. Lefur and P.J. Cozzone 48

66 Simulation of the epidermal growth factor signal transduction pathway
F.A. Brightman, S. Thomas and D.A, Fell 48

67 Computer simulation and evolution strategies in the study of rat heart glucose
metabolism
S. Thomas, M.G. Poolman and D.A. Fell 48

68 A role for the endogenous cannabinoid, anandamide, in the human placenta
1. Das and S.R. Sooranna 48

69 Dexamethasone administration in adulthood leads to cardiac protein kinase C
expression reminiscent of early development
M.L. Langdown, M.J. Holness and M.C. Sugden 49

70 The effect of maternal protein deficiency on the expression of the growth-arrest-
specific gene 6 (gas6) in the fetal kidney
W.D. Rees and S.M. Hay 49

71 Sulphotransferase activity in the human placenta
E.L.Stanley, M.W.H. Coughtrie and R. Hume 49

72 Effect of birth and ambient temperature on abundance of long and short forms of the
prolactin receptor in ovbrown-adipose tissue
J. Bispham, L. Heasman, L. Clarke, P. Ingleton, T. Stephenson and M.E. Symonds 49

73 Developmental changes in the appearance ofleptin in ovine adipose tissue
V. Wilson, L. Heasman, J. Dandrea, T. Stephenson and M.E. Symonds 50

74 Platelet nitric oxide synthase activity and plasma cGMP levels suggests dysregulation
in nitric oxide metabolism in schizophrenia
L Das and N.S. Khan 50

75 Regulation of the hepatic microsomal triglycéride transfer protein (MTP) gene by
dietary cholestérol is maintained in isolated hepatocytes
A.J. Bennett, H.M. Sims, A. Ford, K. Lawler, D.A. White, A.M. Salter and
M.A. Billett 50

76 The effect of oestrogen on the metabolism of chylomicron cholestérol in the rat in vivo
K.M. Botham, M. Avella, A. Cantafora and E. Bravo 50

77 The conversion of chylomicrons of different fatty acid composition into chylomicron
remnants in vivo
M.H. Rahman, M. Avella and K.M. Botham 51

78 Preparation and evaluation of an antibody to rat hepatic lipase: effect on liver
chylomicron remnant uptake
M.S. Lambert, M. Avella, Y.Berhane, E. Shervill and K.M. Botham 51

79 Effects of hypercholesterolaemia on endothelial cell function and chylomicron remnant
uptake by the rat aorta
D.J. Grieve, M.A. Avella, J. Elliott and K.M. Botham 51

80 p-Hydroxycinnamoyl-CoA hydratase/lyase: a Pseudomonas enzyme expressed in hairy
root cultures of Datura stramonium
A. Mitra, M.J. Mayer, A.J. Michael, Y. Kitamura, C. Sewter, A. Narbad, A.J. Parr
and N.J. Walton 51

81 Identification of the amylogenin gene of rice
D.A. Jackson and H.F.J. Bligh 52

82 Solute transport in Methylophilus methylotrophus
J. Mills, N. Wyborn, S. Williams, J. Greenwood and C.W. Jones 52
83 Employment of quinohaemoprotein alcohol dehydrogenase from Gluconobacter sp. 33
entrapped in polypyrrole film for creation of reagentless alcohol biosensor
A. Ramanavicius, W. Schuhmann, K. Habermuller, V. Laurinavicius,
L. Marcinkeviciene, 1. Bachmatova, L. Gorton and E. Csoregi 52

84 Biological fluids alter the surface chemistry of nanostructured silicon semiconductors
S.C. Bayliss and L.D. Buckberry 52

85 The viability of mammalian cells on nanostructured silicon semiconductors
S.C. Bayliss and L.D. Buckberry 53

86 Characterization of the interaction of prothrombin with immobilized phospholipid
membranes
R.R. Patel, J. Patel, K.A. Brown and C. LongstafT 53

87 Immunostimulatory peptides from fish waste hydrolysates
L. Gilmartin, J. Roper, R. Ravallec, A. Gildberg, E. Stenberg, J.E. Harris and L. Jervis 53

89 The effect of A (beta)-amyloid peptides on the uptake of choline and noradrenaline by the
human neuroblastoma SH-SY5Y
N.J. Webster, S. Hodges, C. Peers and P.F.T. Vaughan 53

90 Insulin-stimulated release of glycosylphosphatidylinositol-anchored proteins
S. Pang, S. Movahedi and N.M. Hooper 54

91 Proteolytic fragmentation of aminopeptidase N
L.D. Ofner and N.M. Hooper 54

92 Detergent solubility and proteolytic processing of the prion protein
A.R. Wairnsley, F. Zeng and N.M. Hooper 54

93 Use of neoepitope antibodies to study membrane processing of CD23
T.J. Wright, R.A. Maciewicz and C.R.A. Hewitt 54

94 Sequence analysis and characterization of the rat orthologue of tumour-necrosis-factor-
a-converting enzyme
A.J. Beaumont, J.A. Jury, J. Frayne, R. Devos, J. van der Heyden, L. Jeffrey,
G.J. Price and L. Hall 55

95 Role of intemalization in the regulation of phospholipase-C-coupled receptor
desensitization and resensitization
A. Rac and A.B. Tobin 55

96 The effect of poliovirus infection on mitochondrial function
A. Koundouris, C. Jonson, P.G. Sanders, G.E.N. Kass and M.J. Carter 55

97 Structure and mechanism of the bacterial protein toxin, pneumolysin
R. Gilbert, J. Rossjohn, M. Parker, T. Mitchell, A. Rowe, S. Chen, J. Jiménez,
H. Saibil, 0. Byron and P. Andrew 55

98 Phorbol-ester-induced juxtamembrane cleavage of angiotensin-converting enzyme is
not inhibited by a disulphide-bridged stalk
S.L.U. Schwager, A.J. Chubb, R.R. Scholle, K. Naidoo, W.F. Brandt, C. Eckerskorn,
E.D. Sturrock and M.R.W. Ehlers 56

99 Evolution of hexokinases
M.L. Cardenas, A. Cornish-Bowden and T. Ureta 56

100 Ferredoxin NADP^ reductase: identification of key residues involved in NADPH
binding and electron transfer
C. Leadbeater, D.J. Campopiano, R.L. Baxter and S.P. Webster 56

101 Characterization of ferredoxin (flavodoxin) NADP^ reductase and flavodoxin; key
components of electron transfer in Escherichia coli
L. Mciver, C. Leadbeater, D.J. Campopiano, R.L. Baxter and A.W. Munro 56

102 Photo-induced internal electron transfer in nitrite reductase from Pseudomonas
aeruginosa
E.K. Wilson, A. Bellelli, P. Brzezinski, M. Arose, S. Grasso, S. Liberti, F. Cutnizzola
and M. Brunori 57

103 Cytochrome P-450 BM3: NMR, modelling, mutagenesis and substrate specificity
P. Tsan, C.F. Oliver, S. Modi, W.U. Primrose, M.J. Sutcliffe, L.Y. Lian and
G.C.K. Roberts 57

104 Kinetic analysis of a unique fumarate reductase
M.K. Doherty, S.K. Chapman and G.A. Reid 57

105 Structure-function studies on Arg-289—>Lys mutant flavocytochrome b2
C.G. Mowat, A.D. Pike, S.K. Chapman, G.A. Reid, M. Gondry and F. Lederer 57

106 Cytochromes c from Shewanella putrefaciens NCIMB400
A.E. Hill 58

107 Characterization of L-mandelate dehydrogenase from Rhodotorula graminis
D.J. Robertson 58

108 Engineering non-physiological electron transfer
S.J. Sadeghi and G. Gilardi 58
109 Mitogen-activated protein kinase/extracellular signal-regulated kinase kinase kinase 2
(MEKK2) and MEKK3 activate both mitogen-activated protein kinase kinase 6
(MKK6) and MKK7 in intact cells
K. Deacon and J.L. Blank 58

110 The effect of protein kinase C on dynamin and endocytosis in Schi^osacchromyces
pombe
W.G.C. Horsnell, S. Fletcher and N.T. Goode 59

111 The role of calcium in muscarinic receptor activation of extracellular signal-related
protein kinase and c-Jun N-terminal kinase pathways
P.G. Wylie, R.A.J. Challiss and J.L. Blank 59

112 Vasopressin-induced translocation of SIOO proteins in renal tissue discs in vitro
S. Brant, P. Sharma and A.T. Evans 59

113 The nutritional toxicity of sweet lupin (Lupinus angustifolius) seed proteins
M.H. Rahman 59

_______________________________________

 

BIOCHEMICAL SOCIETY TRANSACTIONS
VOL. 27 , NO. 4 , 1999




X-Ray Structure and Reaction SYOSHIKAWA 351
Mechanism of Bovine Heart
Cytochrome c Oxidase


Small GTPases and Cell c J MARSHALL 363
Cycle Regulation


Using NMR as a Probe of J. P. G. MALTHOUSE 701
Protein Structure and
Function


The Insulin Receptor: from C.MARINO-BUSLJE,M.MARTIN-MARTINEZ 715
K. MIZUGUCHI, K. SIDDLE AND T. L. BLUNDELL
Protein Sequence to
Structure


Phospholipase-induced modulation of rat liver C. E. ROLPH, P. ROBERTS AND S. TAYLOR 371
mixed-function oxidase activity


Adaptive responses to fatty acids are mediated C. N. A. PALMER, M. C. U. GUSTAFSSON, 374
by the regulated expression of cytochromes H. DOBSON, C. VON WACHENFELDT AND
P450 C. R. WOLF


Lipid regulation of gene expression M. C. HUNT AND S. E. H. ALEXSON 378


Sphingomyelin hydrolysis and regulation of the A. H. MERRILL, JR., E. T. MORGAN, 383
expression of the gene for cytochrome P450 M. NIKOLOVA-KARAKASHIAN AND J. STEWART


The cytochrome P450 3A4 inducer M. C. WRIGHT 387
metyrapone is an activator of the human
pregnane X receptor


Identification of intracellular ceramide target M. WICKEL, M. HEINMCH, T. WEBER, 393
proteins by affinity chromatography and TID- J. BRUNNER, M. KRONKE AND S. SCHOTZE
ceramide photoaffinity labelling



Ceramide and apoptosis D. K. PERRY 399


Extracellular actions ofsphingosine I- S. PYNE, S. RAKHIT, A.-M. CONWAY, A. McKiE, 404
phosphate through endothelial differentiation P. DARROCH, R. TATE AND N. PYNE
gene products in mammalian cells: role in
regulating proliferation and apoptosis


Recent advances in the biochemistry of T. KOLTER, T. DOERING, G. WILKENING, 409
glycosphingolipid metabolism N. WERTH AND K. SANDHOFF


Gangliosides and growth factor receptor A. A. RAMPERSAUD, J. L OBUNGER, 415
regulation R.K.PONNAPPAN,R. W. BURRY AND A. J. YATES


Membrane flow, lipid sorting and cell polarity D. HOEKSTRA. M. M. P. ZEGERS AND 422
in HepG2 cells: role of a subapical S. C. D. VAN UZENDOORN
compartment

Mechanisms by which short-chain ceramides D. ALLAN, A. SHAWYER AND A. TAYLOR 428
cause apoptosis


Regulation of sphingolipid and glycosphingolipid A. H. FUTERMAN, S. A. BOLDIN, A. B. BRANN, 432
metabolism during neuronal growth and D. PELLED, I. MEIVAR-LEVY AND K ZISLING
development

colloquium: Lipoprotein(a): the Really Bad Cholesterol?


Lipoprotein(a) in health and disease H. DIEPLINGER 439


Gene structure of apolipoprotein(a) and the B. L KNIGHT 447
regulation of its expression


Role of N-linked glycans, chaperone J. WANG AND A. L WHITE 453
interactions and proteasomes in the
intracellular targeting of apolipoprotein(a)


Use of the West of Scotland Coronary A. GAW, E. A. BROWN. G. DOCHERTY AND 459
Prevention Study bio-bank to provide new I. FORD for the West of Scotland Coronary
insights into the control of plasma Prevention Study Group
lipoprotein(a) concentrations


Genetic polymorphisms of the gene for J.THILLET 463
apolipoprotein(a) and their association with
lipoprotein(a) levels and myocardial infarction


Pharmacological modification of lipoprotein(a) K. SUCKLING 466


colloquium: Glycobiology of Pathogen-Host Interactions

Bacterium-host protein-carbohydrate K.-A. KARLSSON 471
interactions and pathogenicity




Molecular basis of mucin-Pseudomonos R. RAMPHAL 474
interactions



Malaria during pregnancy: parasites, antibodies P. E. DUFFY AND M. FRIED 478
and chondroitin sulphate A


Heparan sulphate and RNA-binding motifs in U. FREVERT 482
the malaria circumsporozoite protein


Role of glycans in Plasmodium folciparum A. BARRAGAN, D. SPILLMANN, J. CARLSON AND 487
infection M. WAHLGREN


Hoemophifus influeniae lipopolysaccharide D. W. HOOD, J. C. RICHARDS AND 493
E. R. MOXON

Glycans in meningococcal pathogenesis M. VIRJI 498


Biosynthesis of the Escherichio coli K5 capsular G. GRIFFITHS, B. BARRETT. N. COOK AND 507
polysaccharide I. S. ROBERTS


Candida cell wall mannosylation: importance in N. A. R. Gow, S. BATES, A. J. P. BROWN, 512
host-fungus interaction and potential as a E. T. BUURMAN, L M. THOMSON AND
target for the development of antifungal drugs C. WESTWATER


Biological role of Tryponosoma cruzf trans- V. L. PEREIRA-CHIOCCOLA AND S. SCHENKMAN 516
sialidase


Proteophosphoglycans from Lehhmania - T. ILG.E: HAN&MAN AND Y-D. STIERHOF - 518
promastigotes and amastigotes


colloquium: Membrane Signalling Complexes

Synaptic targeting and regulation of GABA9A) N. J. BRANDON, F. K. BEDFORD, 527
receptors C. N. CONNOLLY, A. COUVE, J. T. KITTLER,
J. G. HANLEY, J. N. JOVANOVIC, J. UREN,
P. TAYLOR, P. THOMAS, T. G. SMART AND
S. J. Moss

GABA(B)eptors function as heterodimers F.H.MARSHALL,J. WHITE, M. MAIN, A. GREEN 531
AND A. WISE


The N-terminus of RAMPs is a critical S.M.FOORD, A. WISE, J. BROWN. M.J. MAIN 535
determinant of the glycosylation state and AND N. J. FRASER
ligand binding of calcitonin receptor-like
receptor

The molecular architecture of neuronal S. J. TAVALIN, R. S. WESTPHAL, M. COLLEDGE, 539
kinase/phosphatase signalling complexes L K. U\NGEBERG AND J. D. SCOTT


Control of protein phosphatase I in the P. B. ALLEN, L. HSIEH-WILSON, Z. YAN, J. FENG, 543
dendrite C. C. OUIMET AND P. GREENGARD


colloquium: Proteomics

Difference gel electrophoresis M. UNLU 547


A strategy for the characterization of protein A. SHEVCHENKO, W. ZACHARIAE AND 549
interaction networks by mass spectrometry A. SHEVCHENKO

Proteomics in pharmaceutical research and P. CUTLER, H. BIRRELL, M. HARAN, W. MAN, 555
development B. NEVILLE, S. ROSIER, M. SKEHEL AND I. WHITE


colloquium: Quinone-Binding Sites in Membrane Proteins: Structure,
Function and Applied Aspects


Generic features ofquinone-binding sites P. RICH AND N. FISHER 561


Structures of quinone-binding sites in bc(1) E. A. BERRY, Z. ZHANG, L-S. HUANG AND 565
complexes: functional implications S.-H. KIM


Probing the ubihydroquinone primary energy R. E. SHARP, A. PALMITESSA, B. R. GIBNEY, 572
conversion site in the Rhodobocter copsulotus C. C. MOSER AND P. L. DUTTON
cytochrome bCi complex


Famoxadone and oxazolidinones: potent D. B. JORDAN, K. T. KRANIS, M. A. PICOLLELLI, 577
inhibitors of cytochrome be, R. S. SCHWARTZ, J. A. STERNBERG AND
K. M. SUN


Models for structure and function in quinone- L. MURRAY, R. H. PIPES, S. F. HASTINGS AND 581
binding sites: the Escherichio coU quinol W. J. INGLEDEW
oxidase, cytochrome bo(3)


EPR studies of the possible binding sites of the T. OHNISHI, S. MAGNITSKY, L. TOULOKHONOVA, 586
cluster N2, semiquinones, and specific T. YANO, T. YAGI, D. S. BURBAEV AND
inhibitors of the NADH:quinone A.D.VINOGRADOV
oxidoreductase (complex 1)


Quinone-binding sites in membrane proteins: C. R. D. LANCASTER 591
what can we learn from the Rhodopseudomonos
viridis reaction centre?


Properties of the common inhibitor-binding J. G. OKUN, V. ZICKERMANN AND U. BRANDT 596
domain in mitochondrial NADH-
dehydrogenase (complex 1)


Biochemical aspects of N-heterocyclic P. LOMMEN 602
complex-I inhibitors with insecticidal activity


Ubiquinone and inhibitor sites in complex I: M. D. ESPOSTI AND A. GHELLI 606
one, two or three?


Structure and mechanism ofcytosolic quinone M. A. BIANCHET, C. FOSTER, M. FAIG, 610
reductases P. TALALAT AND L. M. AMZEL

colloquium: Phospholipids: Regulators of Membrane Traffic and
Signalling


Structural basis for high-affinity M. A. LEMMON 617
phosphoinositide binding by pleckstrin
homology domains


Phosphoinositide 3-kinase knockout mice: role D. A. FRUMAN, S. B. SNAPPER, C. M. YBALLE, 624
of p85(alfa) in B cell development and proliferation F. W. ALT AND L. C. CANTLEY

Phosphoinositide 3-kinase signalling: no lipids M. P. WYMANN, L. PIROLA, V. L. KATANAEV 629
AND G. BULGARELU-LEVA


Cellular expression and function of N. T. KTISTAKIS, M. MANIFAVA, J. SUGARS, K. BI 634
phospholipase Dl AND M. G. ROTH


ADP-ribosylation factor regulates spectrin A. GODI, I. SANTONE, P. PERTILE, P. MARRA, 638
skeleton assembly on the Golgi complex by G. Di TULUO, A. LUINI, D. CORDA AND
stimulating phosphatidylinositol 4,5- M. A. DE MATTEIS'
bisphosphate synthesis


ADP-ribosylation factor GTPases in signal D. JONES, B. BAX AND S. COCKCROFT 642
transduction and membrane traffic:
independent functions ?


Generation of phosphatidylinositol-specific C. L THOMAS, J. STEEL, G. D. PRESTWICH AND 648
antibodies and their characterization G. SCHIAVO


Phospholipase C(delta) and related molecules H. YAGISAWA, M. Fujii AND M. HIRATA 652


The type II PIPkins (Ptdlns5P 4-kinases): K. A. HINCHLIFFE, A. CIRUELA, J. A. MORRIS. 657
enzymes in search of a function? N. DIVECHA AND R. F. IRVINE


Regulation of early-endosome dynamics by M. J. CLAGUE, A. T. JONES, I. G. MILLS, 662
phosphatidylinositol 3-phosphate binding D. M. WALKER AND S. URBE
proteins


Regulation of endocytic membrane traffic by J.-M. GAULLIER, D. GILLOOLY, A. SIMONSEN AND 666
phosphatidylinositol 3-phosphate H. STENMARK


Lipid kinases and trons-Golgi network S. PONNAMBALAM, S. CLOUGH, C. P. DOWNES, 670
membrane dynamics J. M. LUCOCQ, H. J. MCLAUCHLAN AND
M. C. TOWLER


Phosphatidylinositol 3,5-bisphosphate: a novel S. K. DOVE, R. K. McEwEN. F. T. COOKE, 674
lipid that links stress responses to membrane P. J. PARKER AND R. H. MICHELL
trafficking events


Divergent signalling mechanisms involved in L M. FLETCHER AND J. M. TAVARE 677
insulin-stimulated GLUT4 vesicle trafficking to
the plasma membrane


Potential regulation of ADP-ribosylation factor P. J. CULLEN AND K. VENKATESWARLU 683
6 signalling by phosphatidylinositol 3,4,5-
trisphosphate


colloquium: Molecular Interactions in Complex Proteins and Nucleic
Acids


The assembly of the EcoKI type I DNA D. T. F. DRYDEN. G. D. DAVIES, I. MARTIN, 691
restriction/modification enzyme and its L M. POWELL. N. E. MURRAY, D. J. ELLIS, T. BERGE,
interaction with DNA J. M. EDWARDSON AND R. M. HENDERSON


Restriction endonuclease reactions requiring S. E. HALFORD, D. T. BILCOCK, N. P. STANFORD, 696
two recognition sites S. A. WILLIAMS, S. E. MILSOM, N. A. GORMLEY,
M. A. WATSON, A. J. BATH, M. L EMBLETON,
D. M. GOWERS, L. E. DANIELS, S. H. PARRY AND
M. D. SZCZELKUN




Peptide synthesis with modified trypsin S. GREEN AND C. 0 FAGAIN 727


Generation of non-bitter casein hydrolysates by G. O'CuiNN, R. FITZGERALD, P. BOUCHIER AND 730
using combinations of a proteinase and M. MCDONNELL
aminopeptidases


Role of matrix metalloproteinases in normal S. MCDONNELL, M. MORGAN AND C. LYNCH 734
and disease processes


The cathepsin L-like proteinases of liver fluke C. P. BRADY, A. J. DOWD, J. TORT, L. ROCHE, 740
and blood fluke parasites of the trematode B. CONDON, S. M. O'NEILL, P. J. BRINDLEY AND
genera Fasciola and Schistosoma J. P. DALTON


The expanding superfamily ofserpins: D. M. WORRALL, O. E. BLACQUE AND 746
searching for the real targets R. C. BARNES





 

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BIOCHEMICAL SOCIETY TRANSACTIONS

VOL. 27, NO. 5, 1999

MORTON LECTURE


El The need for expression expertise in solid-state NMR studies of membrane protein and
peptides: successes and wish lists
A. Watts 131

SPEAKER ABSTRACTS

SOCIETY SPECIAL COLLOQUIUM
Structure and Function of A-Domains
Speaker Session Time: Thursday 22 July, 09.15 - J6.45

A1 Structural studies of the integrin a2-l domain
J. Ernsley 131

A2 Structure and function of von Willebrand factor type A (vWF-A) domains in
complement and coagulation
S.J. Perkins 131

A3 Functional role ofA-domains in type VI collagen
C.A. Shuttleworth 131

A4 The matrilins: a growing family ofA-domain-containing proteins
M. Paulsson 132

A5 Integrin I-domains and their function
B. Leitinger 132

A6 The A-domain of a malaria protein mediates infectivity
M.R. Hollingdale 132

A7 Vitrin: the second member of a new branch of the von Willebrand factor type A
(vWF-A) superfamily
R. Mayne 132

A8 The evolution of A-domains
D.S. Tuckwell 133

HOST COLLOQUIUM
Molecular Control ofApoptosis

Speaker Session Times: Tuesday 20 July, 09,00 - 15.30
Wednesday 21 July, 09,15-15.30

B1 Control of apoptosis in cell-free systems
P.R. Clarke 133


B2 Biochemical mechanisms of nuclear changes during apoptotic execution
K. Sainejima 133


B3 Caspases and commitment to cell death
V.L. Hedge 133


B4 Mechanisms of neutrophil apoptosis and the regulation of inflammation
M. Whyte 134

B5 Epstein-Barr virus (EBV) and apoptosis: viral mimicry of cellular pathways
L.S. Young 134

B6 Death receptors, nuclear factor (NF)-KB activation and apoptosis: the potential for
therapeutic intervention
S.N. Farrow 134

B7 Poxvirus strategies to prevent apoptosis of infected cells
G.L. Smith 134

B8 Apoptosis and the MHC
T. Rich 135

B9 T cell apoptosis: mechanism and consequences
H.J.M. Brady 135

BIO Bcl-2 related proteins and cancer
G.PackhamL 135


Bll c-Jun and Bax: regulators of programmed cell death in developing neurons
J. Ham 135


B12 Suppression of apoptosis by v-AbI protein tyrosinekinase
C. Dive 136


HORMONE GROUP COLLOQUIUM
Gene Therapy: from Bench to Bedside

Speaker Session Times: Tuesday 20 July, 09.00 - 16.10
Wednesday 21 July, 09.10 - 12.50

Cl Lentivirus vectors for gene therapy
A.M.L. Lever 136

C2 Favourable expression and safety profiles with "gutless" adenoviral vectors
S. Kochanek 136


C3 Adeno-associated virus vectors; gene therapy and viral vectors, the gap is closing
R.J. Samulski 136



C4 Herpes virus vectors for gene therapy in the nervous system
D.S. Latchman 137


C5 Towards gene therapy for cystic fibrosis
C.F. Higgins 137


C6 Indefinite heart allograft survival after adenovirus-mediated gene transfer of
transforming growth factor (TGF)pH or CTLA4
I. Anegon 137


C7 Immunotherapy of autoimmune disease by gene transfer
Y. Chemajovsky 137


C8 The basic science of brain tumour gene therapy: advantages and challenges ahead
P.R. Lowenstein 138


C9 Gene transfer approaches for the treatment of neurodegenerative diseases
P. Aebischer 138

C10 Clinical trials of brain glioblastoma using retroviral vectors
D. Klatzmann 138

Cll Gene delivery to the central nervous system (CNS) using non-viral vectors
L.W. Seymour 138


C12 Targeting the hypothalamus: curing salt loss in Brattleboro rats
J.B.Uney 139

C13 Cell-type-specific expression in the pituitary: physiology and cancer treatment
M.G. Castro 139


C14 Genetic approaches to vaccination for lymphoma
R.E. Hawkins 139

MEMBRANE GROUP/MOLECULAR AND CELLULAR PHARMACOLOGY
GROUP

Expression and Purification of Membrane Proteins

Speaker Session Times: Wednesday 21 July, 08.45 - 16.55
Thursday 22 July, 09.10 - 16.25

Dl Membrane protein biosynthesis at the endoplasmic reticulum
S. High 139

D2 Expression and purification of the mitochondrial uncoupling proteins (UCPs): a
comparative study between Escherichia coli and Saccharomyces cerevisiae
B. Miroux 140


D3 Expression ofprokaryotic membrane transport proteins in Escherichia coli: successes
and failures
A. Ward 140

D4 Functional expression in Escherichia coli and purification of a G-protein-coupled
receptor for neurotensin
R. Grisshammer 140

D5 Overexpression, refolding and crystallization of an 80 kDa outer membrane iron
transporter from the outer membrane of Escherichia coli
S.K. Buchanan 140



D6 Refolding of G-protein-coupled receptors from inclusion bodies produced in
Escherichia coli
H. Kiefer 141

D7 Amplified expression and membrane reconstitution of transport protein
B. Poolman 141


D8 Characterization and purification of a plant sucrose transporter produced in
Saccharomyces cerevisiae
J. Stolz 141

D9 Heterologous expression of the human red-cell anion exchanger (Band-3; AEI)
J.D. Groves 141

D10 Purification and crystallization of His6-tagged membrane proteins produced in
transgenic tobacco
R. Flachmann 142

Dl1 The use of baculovirus vectors for the production of membrane proteins in insect cells
R.D. Possee 142


D12 Improving the expression of the serotonin transporter in insect cells by co-expression
of molecular chaperones
C.G. Tate 142

D13 Recombinant baculovirus-based functional expression of visual pigments:
optimalization of expression level, purification and reconstitution
W.J. DeGrip 142

D14 Heterologous expression ofnicotinic acetylcholine receptors in mammalian and
Drosophila cell lines: the importance of the host cell environment
N.S. Millar 143

D15 Expression and purification of rhodopsin and its mutants from stable mammalian cell
lines for NMR studies
P.J. Reeves 143

D16 Expression of seven transmembranc receptors in mammalian cells
M. Needham 143


D17 Transient expression technologies, their application and scale-up: 5-
hydroxytryptamine-3 (5HT-3) receptor case study
H. Blasey 143

SOCIETY SPECIAL COLLOQUIUM
Structure and Function ofA-Domains

Poster Session Times: Tuesday 20 July, 17.00-1830
Wednesday 21 July. 17.00 - 1830
Thursday 22 July, 10.00 - 11.00

1 Investigating the metal-ion-dependent adhesion site (MIDAS) function in collagen VI
assembly
C. Baldock, H. Fakhoury, S.G. Ball, C.A. Shuttleworth and C.M. Kicity 144


2 In vitro assembly of type VI collagen
S.G. Ball, C.M. Kielty and C.A. Shuttleworth 144


3 The collagen sequence, GFOGER, is a binding site for integrin ex I and a2 A-dornains
and fully mediates a2p I-dependent cell recognition by collagen
C.G. Knight, L.F. Morton, A.R. Peachey, D.S. Tlickwell, R.W. Parndale and M.J. Barnes 144


4 Identification of the C3B binding site in the recombinant von Willebrand factor type-A
(vWF-A) domain of complement factor B by laser desorption/ionization MS and
homology modelling
J. Hinshelwood, D.I.R. Spencer and S.J. Perkins 144


5 The A-domain of integrin aEB7 is involved in binding to E-cadherin
J.M.G. Higgins, M. Cernadas and M.B. Brenner 145

HOST COLLOQUIUM
Molecular Control of Apoptosis

Poster Session Times: Tuesday 20 July, 17.00- 18.30
Wednesday 21 July. 17.00 - 18.30
Thursday 22 July, 10.00-11.00

6 Anti-hepatotoxic activity ofcaffeic acid: a phenolic compound from Artemisia
scoparia
A.H. Gilani, K.H. Janbaz and S.A. Saced 145

7 Jun protein is involved in suppression of apoptosis during herpes simplex virus type-1
infection
G. Zachos and J. Conner 145


8 Inhibition of apoptosis by nitric oxide donors in guinea-pig gastric mucous cells
K. Johal, C.L. Potter and P.J. Hanson 145

9 4-Hydroxylphenyl retinamide-induced apoptosis in squamous cell carcinoma cells of
vulva: role of Ca2+
E. Ulukaya and E.J. Wood 146

10 Nitric-oxide-induced cell death of PC 12 cells
A. Price and G.C. Brown 146

11 Effects of nitric oxide on activity of caspases in vitro and in macrophages
V. Borutaite and G.C. Brown 146

12 The Golgi fragments during apoptosis
J.G. Pryde and A. Walker 146

13 Micro-environmental survival stimuli combine to regulate Bcl-X and Bax and suppress
drug-induced apoptosis in B lymphoma cells
S.T. Taylor, J.A. Hickman and C. Dive 147

14 v-Abl-mediated up-regulation of p21(WAF-1) in growth-arrested and proliferating myeloid
cells
S. Khanna, R. Brown, K. Ball and C. Dive 147

15 Activation of v-AbI tyrosine kinase suppresses apoptosis and regulates
phosphorylation of protein kinase B (PKB) and the pro-apoptotic protein Bad
D. James and C. Dive 147


16 Isolation and characterization ofEpstein-Barr virus (EBV) BHRFI homologues from
Herpesvirus papio
T. Williams and S. Hazlewood 147


17 Isolation and characterization of apoptosis-controlling genes from primate
herpesviruses
M. Howell and S. Hazlewood 148


HORMONE GROUP COLLOQUIUM
Gene Therapy: from Bench to Bedside

Poster Session Times: Tuesday 20 July, J7.00 - 18.30
Wednesday 21 July, 17.00 - 18.30
Thursday 22 July, 10.00 - 11.00


18 Poster withdrawn
148

19 A physiologically regulated adenoviral vector for the treatment ofischaemic disease
K. Binley, L. Griffiths, S. lqball, H. Spearman, S.M. Kingsman, A. Kingsman and S. Naylor 148


20 Development of retroviral transduced haematopoietic stem cells towards a novel
approach to cancer gene therapy
J.S. Freeth, S. lqball, J. Rohll, S. Watt, C.E. Lewis, S.M. Kingsman and S, Naylor 148


21 A gene transfer strategy to manipulate nitric oxide in the vasculature of a hypertensive
rat model
M.Y. Alexander, M.J. Brosnan, J. Fennell, A.M. Deviin, C.A. Hamilton and A.F. Dominiczak 149

MEMBRANE GROUP COLLOQUIUM
Expression and Purification of Membrane Proteins

Poster Session Times: Tuesday 20 July, 17.00 - 18.30
Wednesday 21 July, 17.00 - 18.30
Thursday 22 July, 10.00 - 11.00

22 Comparative study of purine transporters in drug-sensitive and drug-resistant
Trypanosoma equiperdum
J.E. Leadsham and S.M. Jarvis 149


23 Transport of purine nucleobases by COS-1 cells occurs via a nitrobenzylthioinosine-
insensitive (ei) nucleoside transporter
D. Johnson and S.M. Jarvis 149


24 Characterization of purine transporters in L6 rat skeletal-muscle cells
H.L. Maddock, S. Vine, J.D. Pearson and S.M. Jarvis 149

25 Expression of isotopically labelled membrane transport proteins
H. Venter, R.B. Herbert, P.J.R. Spooner, A. Watts and P.J.F. Henderson 150

26 Overexpression, purification and structural analysis of the Escherichia coli L-fucose-H+
membrane transport protein, FucP
J. Clough and P.J.F. Henderson 150


27 In vitro expression and metabolism ofpresenilin-I protein
C. Houghton, J. Arnold, M. Shearman, S. Dawson, M. Landon, R.J. Mayer and R. Layficid 150



28 Multiplex TaqMan(r) combined reverse transcriptase (RT)-PCR: a novel method for the
quantification ofmRNA levels in receptor-transfected mammalian cell clones
P.R. Murdock, K.B. Tan, N.C. Herrity, G.L Rennie and 0. Jenkins 150


29 Electrochemical detection of secreted placental alkaline phosphatase
E. Kelso, J. McLean and M. Cardosi 151


30 Optimization of human fi opioid receptor expression in baculovirus-infected insect
cells
D. Massotte, L. Baroche, C. Pereira, T. Suply, B. Perret, B. KiefFer and F. Pattus 151

31 Expression of human a2C2-adrenergic receptor in different host-vector systems
H. Heimo, V.-P. Jaakola, A. Kapat, P. Heikinheimo, M. Rantanen, T. Glurnoffand A. Goidman 151

BIOCHEMICAL IMMUNOLOGY GROUP

Poster Session Times: Tuesday 20 July, 17.00- 18.30
Wednesday 21 July. 17.00- 18.30
Thursday 22 July, 10.00 - 11.00


32 Effect of rabbit lgG anti-xanthine oxidoreductase antibodies on NADH and oxidase
activities of human and bovine xanthine oxidoreductase
H. Djellili, F. Larguet, L. Arrar and M. Benboubetra 151

33 Studies on semen and anti-sperm antibodies in male infertility
N. Alam and M.A. Rahman 152

MOLECULAR ENZYMOLOGY GROUP

Poster Session Times: Tuesday 20 July, 17.00 - 18.30
Wednesday 21 July, 17.00 - 18.30
Thursday 22 July, 10.00 - 11.00

34 Kinetic inhibition studies of xanthine oxidase activity of both human and bovine milk
xanthine oxidase (XO) by allopurinol, alloxanthine and uric acid
F. Larguet, H. Djellili, A. Baghiani and M. Benboubetra 152

NEUROSCIENCE GROUP
Poster Session Times: Tuesday 20 July, 17.00- 18.30
Wednesday 21 July, 17.00 - 18.30
Thursday 22 July, 10.00 - 11.00

35 Astrocytes release and preserve antioxidants: implications for neuroprotection
R. Stone, V.C.Stewart,R.D. Hurst, J.B. dark and S.J.R.Heales 152




NUCLEIC ACIDS AND MOLECULAR BIOLOGY GROUP

Poster Session Times: Tuesday 20 July, 17.00-18.30
Wednesday 21 July. 17.00-18.30
Thursday 22 July, 10.00 - 11.00

36 Selective inhibition ofphlebo-, retro-, flavo- and pox-viruses by nucleoside and PFA
analogues
J.M. Bailey and K. Nelson 152

PROTEIN AND PEPTIDE SCIENCE GROUP

Poster Session Times: Tuesday 20 July, 17.00-18.30
Wednesday 21 July, 17.00 - 18.30
Thursday 22 July, 10.00 - 11.00

37 The decrease of tabtoxin produced by Pseudomonas fabaci in batch culture
D. Harzallah and L. Larous 153

Speakers' papers will be published in Biochemical Society Transactions
volume 27, part 6 (December 1999)


 

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