BIOTECHNIQUES
VOL. 26, NO. 6, JUNE 1999
Benchmarks
Differential Display Combined with a Regulated Transient
Expression System
H. Kanamori and D.J. Shapiro 1018
Effect of Growth Temperature and Vector Ends Phosphorylation
in the Cloning Efficiency of Specific DNA Fragments
A.R. Sepulveda and M.W. Lieberman 1022
Instability of a Multiple Copy Enhancer in Plasmid Vectors:
Practical Considerations
D.J. Discher, N.H. Bishopric, O.M. Hemandez and K.A. Webster 1026
Functional Testing of a Bicistronic Retroviral Vector for Intracellular
Peptide Production
M. Duch, A. Tolstrup, 1. Dalum, T. Jespersen, S. Mouritsen and F.S. Pedersen 1032
Vectors for Inducible Expression of Dual Epitopc-Tagged Proteins in
Insect Cells
H. Braun and G. Suske 1038
Screening Procedure for Pichiapastoris Clones Containing Multiple
Copy Gene Inserts
W.D. Fairlie, P.K. Russell, H.P. Zhang and S.N. Breit 1042
Direct Cloning of Differential Display Products Eluted from
Northern Blots
E.M. Denovan-Wright, S.E. Howlett and H.A. Robertson 1046
Simple Protocol for Extracting Nuclear DNA from Single Embryos
of a Marine Snail
R.J. Simpson, C.S. Wilding andJ. Grahame 1050
Extracting High-Quality DNA from Shed Reptile Skins:
A Simplified Method
J.W. Fetzner, Jr. 1052
Recycling of Anion-Exchange Resins for Plasmid DNA Purification
V.W.-H. Chang, R. Wii and Y.-S. Ho 1056
Removal of Ethidium Bromide and Cesium Chloride from Plasmid
DNA by Ethanol Precipitation
J. Song, 1. Kanazawa, K. Sun, T. Murata and K.K. Yokoyama 1050
Color Coding the Cell Death Status of Plant Suspension Cells
T. Shigaki and M.K. Bhattacharyya 1060
Morphological Detection of Plasma Membrane Changes During
Apoptosis Using Enhanced Green Fluorescent Protein
E. Shinbrot, C.M. Spencer and S.R. Kain 1064
Extending the Useful Life Span of DNA Probes for Fluorescence
In Situ Hybridization
A.T. Christian, H.E. Garcia and J.D. Tucker 1068
Microtiter Immunocytochemical ELISA Assay
S.M. de la Monte, N. Ganju and J.R. Wands 1073
Improved Protocol for Using Avian Red Blood Cells as Substrates
for the Polymerase Chain Reaction
D. Bercovich, Y. Plotsky and Y. Gruenbaum 1080
Bridge-Overlap-Extension PCR Method for Constructing
Chimeric Genes
R.K. Mehta and J. Singh 1002
Microsatellite Locus Amplification Using Deer Antler DNA
A. O'Connell and R.M. Denome 1086
Internet On-Ramp
Snapping Up SNPs
S.M. Brown 1090
Short Technical Reports
Differential Sensitivity to 5-fluoro-orotic Acid as a Screen for Bait
RNA-Independent False Positives in a Yeast Three-Hybrid System
Y.W. Park, S.-L. Tan and M.G. Katze 1102
Rapid Genetic Screening for Hemochromatosis Using Automated
SSCP-Based Capillary Electrophoresis (SSCP-CE)
A.-K. Bosserhoff, S. Seegers, C. Hellerbrand, J. Scholmerich and R. Buttner 1106
Absolute Quantitation of MDRI Transcripts Using Heterologous
DNA StandardsValidation of the Competitive RT-PCR
(CRT-PCR) Approach
A. EI-Osta, P. Kantharidis andJ. Zaicberg 1114
Model System for Plant Cell Biology: GFP Imaging in Living Onion
Epidermal Cells
A. Scott, S. Wyatt, P.-L. Tsou, D. Robertson and N. Stromgren Alien 1125
Research Reports
Fast and Reliable Screening of Mutations in Human Thmors: Use of Multiple
Fluorescence-Based Long Linker Arm Nucleotides Assay (mf-LLA)
L.A. Marcelino, M. Galvin, G.M. Martins, M.J. Proenca, E. Mayrand,
J.A. Rueffand C.J. Monteiro 1134
Construction of Gene Targeting Vectors from A,KOS Genomic Libraries
S. Wattler, M. Kelly and M. Nehis 1150
BioComputing
Identification of Relative Protein Bands in Polyacrylamide Gel
Electrophoresis (PAGE) Using a Multi-Resolution Snake Algorithm
M.N. Gurcan, M. Koyutiirk, H.S. Yildiz, R. Cetin-Atalay and A.E. cetin 1162
WinGene/WinPep: User-Friendly Software for the Analysis of
Amino Acid Sequences
L. Hennig 1170
Digital Image Processing for Rapid Analysis ofDiffercntially
Expressed Transcripts on High-Density cDNA Arrays
B. Adryan, V. Cariguth and H.J. Decker 1174
Spreadsheet-Based Program for Alignment of Overlapping
DNA Sequences
R. Anbazhagan and E. Gabrielson 1180
ScienceLabDatabase: A Computer Program to Organize a
Molecular Biology Laboratory
0. Nayler and S. Stamm 1186
1999 Author Index (Volume 26) 1192
New Products 1196
Index to Advertisers 1207
____________________________________________
BIOTECHNIQUES
VOL. 26, NO. 5, MAY 1999
Benchmarks
Extender PCR: A Method for the Isolation of Sequences Regulating
Gene Expression from Genomic DNA
A.J.H. Brown, S.J. Perry, S.E. Saunders andJ.R Burke 804
Baculovirus-Transfer Vector for Eukaryotic Expression and
Immunoaffinity Purification of Gal4-Fusion Proteins 808
G. Chen and A.J. Courey
Generation of Epitope-Tagged Proteins by Inverse PCR Mutagenesis
L. Gama and G.E. Breitwieser 814
Selective PCR Amplification of Functional Immunoglobulin Light Chain
from Hybridoma Containing the Aberrant MOPC 21-Derived VK by
PNA-Mediated PCR Clamping
O. Cochet,E. Martin, W.H.Fridman and J.-L.Teillaud 818
The pKNOCK Series of Broad-Host-Range Mobilizable Suicide Vectors
for Gene Knockout and Targeted DNA Insertion into the Chromosome
of Gram-Negative Bacteria
M.F. Alexeyev 824
Amplification of FADDmRNA Using RT-PCR Results in an Artifact
M. Alonso and R.L. Pastori 828
Concentration ofRecombinant Baculovirus by Cation-Exchange
Chromatography
J. Barsourn 834
Improved LM-PCR Procedure for In Vivo Footprinting Analysis of
GC-Rich Promoters
L. Le Cam, J. Polanowska, L. Fajas, E. Fabbrizio and C. Sardet 840
Reverse Strand Priming: A Versatile cDNA Radiolabeling Method for
Differential Hybridization on Nucleic Acid Arrays
W. Mikulits, H. Dolznig, R. Hofbauer and E.W. Mullner 846
Nonselective Colony-Color Assays for HIS3, LEU2, LYS2, TRPI and
URA3 in ade2 Yeast Strains Using Media with Limiting Nutrients
T.A. Myers andJ.A. Nickoloff 850
Modification of Enzyme-Conjugated Streptavidin-Biotin Western Blot
Technique to Avoid Detection of Endogenous Biotin-Containing Proteins
G.M. Vaitaitis, R.J. Sanderson, E.J. Kimble, N.D. Elkins and S.C. Flores 854
Mapping of Nuclear Localization Signals by Simultaneous Fusion to
Green Fluorescent Protein and to p-Galactosidase
G. Sorg and T. Stamminger 858
Induction of Cell Death by Hydrophobie Hydrocarbons
T.A. Kelly, M.S. Felder and R.A. Ollar 864
Microprep Method for Rapidly Isolating Plasmid DNAs for
Restriction Enzyme Analysis
A.R. Shepard and J.L. Rae 868
Simple Version of "Megaprimer " PCR for Site-Directed Mutagenesis
A. Colosimo, Z. Xu, G. Novelli, B. Dallapiccola and D.C. Gruenert 870
Internet On-Ramp
Scripting Wizards for Chime(™ )and RasMol
R.M. Horton 874
Identification and Isolation ofDifferentially Expressed Genes from
Very Small Tissue Samples
P. Gonzalez, J.S. Zigler, Jr., D.L. Epstein andT. Borras 884
Increasing DNA Transfer Efficiency by Temporary Inactivation of
Host Restriction
R.A. Edwards, R.A. Helm and S.R. Maloy 892
Microsatellite Analysis Using a Two-Step Procedure for Fluorescence
Labeling ofPCR Products
S. Jouquand, A. Cheron and F. Galibert 902
Internal and Flanking Sequence from AFLP Fragments Using
Ligation-Mediated Suppression PCR
J.M. Schupp, L.B. Price, A. Klevyska and P. Keim 905
Research Reports
Two-Color GFP Expression System for C. elegans
D.M. Miller I I I N.S. Desai, D.C. Hardin, D.W. Piston, G.H. Patterson,
J. Fleenor, S. Xu and A. Fire 914
Use of Enhanced Green Fluorescent Protein to Optimize and Quantitate
Infection of Target Cells with Recombinant Retroviruses
L.M. Cashion, L.A. Bare, S. Harvey, Q. Trinh, Y. Zhu and J.J. Devlin 924
Detection of Virtually All Mutations-SSCP (DOVAM-S): A Rapid
Method for Mutation Scanning with Virtually 100% Sensitivity
Q. Liu, J. Feng, C. Buzin, C. Wen, G. Nozari, A. Mengos, V. Nguyen,
J. Liu, L. Crawford, F.K. Fujimura and S.S. Sommer 932
Production of Human CNS Neurons from Embryonal Carcinoma
Cells Using a Cell Aggregation Method
W.M.W. Cheung, W.Y. Fu, W.S. Hui and N.Y. Ip 946
Determination of Phage Antibody Affinities to Antigen by a
Microbalance Sensor System
A. Hengerer, C. Kosslinger, J. Decker, S. Hauck, 1. Qlieitsch,
H. Wolf and S. Dübel 956
High-Performance Subtractive Hybridization ofcDNAs by Covalent
Bonding Between Specific Complementary Nucleotides
S.-Y. Ying and S.Lin 966
Product Application Focus
Automated System for Purification of Dye-Terminator Sequencing
Products Eliminates Up-Stream Purification of Templates
B.M. Fangan, O.J. Dahiberg, A.H. Deggerdal, M. Bosnes and F. Larsen 980
GelStar® Nucleic Acid Gel Stain: High Sensitivity Detection in Gels
H.W. White, N.B. Vartak, T.G. Burland, F.P. Curtis and N. Kusukawa 984
New Products 990
Index to Advertisers 999
_______________________________________
BIOTECHNIQUES
VOL. 27, NO. 1, JULY 1999
Benchmarks
Heteroduplex Cleavage Analysis Using SI Nuclease
J.T. Howard, J. Ward, J.N. Watson and K.H. Roux 18
Improvement ofSSCP Analysis by Use ofDenaturants
S.P. Yip, D.A. Hopkinson and D.B. Whitehouse 20
Subcellular Fractionation of Group B Streptococcus
D.E. Kling, L.C. Madoffand J.L. Michel 24
Use of GFP as a Reporter for the Facile Analysis of Sequence-
Specific Proteases
J.C. Pehrson, A. Weatherman, J. Markwell, G. Sarath and
S.D. Schwartzbach 28
Cost-Effective Staining ofDNA with SYBR® Green in PreparativeAgarose Gel Electrophoresis
S.E. Miller, P. Taillon-Miller and P.-Y. Kwok 34
Prevention of Anomalous Band Mobility in A+G Sequencing
Ladders of3'-End-Labeled DNA
S. Starrs and R.J.H. Davies .............................. . 36
System for PCR Identification ofcDNA Ends (SPICE)
D.R. Koehier, R. Tellier, J. Hu and M. Post ..................... . 46
Transfer RNA Reduces the Formation of Primer Artifacts During
Quantitative PCR
S.R. StUrzenbaum .................................... 50
New Series otDrosophila Expression Vectors Suitable for
Behavioral Rescue
G. Roman, J. He and R.L. Davis ........................... . 54
S.. pombe Expression Vector with 6x(His) Tag for Protein Purification
and Potential for Ligation-independent Cloning
T. Hosfield and Q. Lu 58
Black Cellular Spreading and Motility Assay
A.-E. Al Moustafa, N. Urbani and M. O'Connor-McCourt 60
Alternative Procedure for Two-Dimensional Separation of
Phosphoamino Acids
C. Grangeasse, M. Riberty, E. Vaganay and B. Dliclos 62
Rapid DNA Extraction from Ferns for PCR-Based Analyses
E.L. Dempster, K.V. Pryor, D. Francis, J.E. Young and H.J. Rogers 66
Rapid, Economical Filter Alternative to Chromosomal DNA
Centrifugation in the Alkaline Lysis Plasmid Protocol
M.T. McNally ...................................... 68
Large-Scale Preparation of Sequence-Ready Bacterial Artificial
Chromosome DNA Using QIAGEN® Columns
L.S. Kirschner and C.A. Stratakis .......................... . 72
Internet On-Ramp
Biological Sequence Analysis Using Regular Expressions
R.M. Horton ....................................... 76
Short Technical Reports
Application of the Green Fluorescent Protein as a Reporter for
Acel-Based, Two-Hybrid Studies
G. Mayer, H. Launhardt and T. Munder 86
Generation of Vector-Insensitive Dig-Labeled Probes from Large
Cosmid Library Inserts Using PCR
E. Morgan, J.M. Lodge, J.Stephen and N.L. Brown . 94
Eukaryotic Conditional Expression System
T. Sourisseau, Y. Le Drean, G. Slabert, P. Flamant and D. Michel . 106
Purification of Proteins Fused to Either the Amino or Carboxy
Terminus of the My cobacterium xenopi Gyrase A Intein
M.W. Southworth, K. Amaya, T.C. Evans, M.-Q. Xu and F.B. Perler 110
Rapid Quantitation of Gene Therapy Specific CFTR Expression
Using the Amplification Refractory Mutation System
P.H. Thorpe and D.J. Porteous 122
Research Reports
Detection of Low-Frequency Mutations in Exon 8 of the TP53 Gene
by Constant Denaturant Capillary Electrophoresis (CDCE)
P.O. Ekstr0m, A.-L. B^rresen-Dale, H. Qvist, K.E. Giercksky
and W.G. Thilly ................................... . 128
Absolute Quantitation of Specific mRNAs in Cell and Tissue
Samples by Comparative PCR
A.M. Martorana, G. Zheng, F. Springall, HJ. Hand, R.L. O'Grady
and J.G. Lyons 136
Chemiluminescent Detection of Sequential DNA Hybridizations to
High-Density, Filter-Arrayed cDNA Libraries: A Subtraction
Method for Novel Gene Discovery
D. Guiliano, M. Ganatra, J. Ware, J. Parrot, J. Daub, L. Moran, H. Brennecke,
J.M. Foster, T. Supali, M. Blaxter, A.L. Scott, S.A. Williams and B.E. Slatko 146
Temporal, Spatial and Tissue-Specific Expression of a Myogenin-lacZ
Transgene Targeted to the Hprt Locus in Mice
J.L. Vivian, W.H. Klein and P. Hasty ....................... 154
pSURF-2, A Modified BAG Vector for Selective YAC Cloning and
Functional Analysis
A.C. Boyd, H. Davidson, B. Stevenson, G. McLachlan,
H. Davidson-Smith and D.J. Porteous 164
Product Application Focus
Automated System for Capture and Detection ofNucleic Acids
A. Brown, A.A. Akinsanya, S.J. Barker, M. Brophy, A.K. Dobb,
S.M. Doyle, l.R. Hudson, S.J. Minter, M.J. Wraith and J.D. Oultram .. . .. . 176
Literature Express................................ 182
New Products ................................... 189
Index to Advertisers ............................ .. 198
_______________________________________
BIOTECHNIQUES
VOL. 27, NO. 2, AUGUST 1999
Benchmarks
Luciferase Reporter Gene Vectors that Lack Potential AP-I Sites
S.L. Grimm and S.K. Nordeen ............................ . 220
Adapter-Aided PCR to Identify T-DNA Junctions in
Transgenic Plants
Y.I. Choi, E.W. Noh, M.S. Han and S.H. Son ....... 222
Gene Dosage by Quantitative Real-Time PCR
J.-L. Bolilay, J. Reuter, R. Ritschard, L. Terracciano, R. Herrmann
and C. Rochlitz 228
High-Speed Prothrombin G->A 20210 and Methylenetetrahydrofolate
Reductase C->T 677 Mutation Detection Using Real-Time
Fluorescence PCR and Melting Curves
C. Aslanidis, M. Nauck and G. Schmitz 234
Thermal Asymmetric Interlaced PCR Amplification ofYAC Insert
End Fragments for Chromosome Walking in Plasmodium
falciparum and Other A/T-Rich Genomes
J.M. Foster, Z. Christodoulou, G.M. Cowan and C.I. Newbold ........... . 240
Hydrogen Peroxide Improves the Efficiency of a Peripheral Blood
PCR Assay for Diagnosis of Human Brucellosis
M.I. Queipo-Ortuno, M.A. Garcia-Ordonez, J.D. Colmenero
and P. Morata 248
UV Light Irradiation of Plastic Reaction Tubes Inhibits PCR
L.C. Burgess and J.O. Hall 252
Specific Requirements for PCR Amplification of Long Mitochondrial
A+T-Rich DNA
J.C. Rondan Duenas, G.M. Panzetta-Dutari and C.N. Gardenal 258
Identifying Mutations in Drosophila Genes by Direct Sequencing
of PCR Products
E.M. Schlag and D.A. Wassarman 262
DGGE is More Sensitive for the Detection of Somatic Point
Mutations than Direct Sequencing
B. TrUIzsch, K. Krohn, P. Wonerow and R. Paschke 266
High-Resolution, Fluorescence-Based Differential Display on a
DNA Sequencer Followed by Band Excision
B. Reinhardt, U. Frank, K. Gellner, J.U. Lohinann and T.C.G. Bosch 268
DNA Analyses from Biological Material Previously Used for
Scanning Electron Microscopy Studies
S. Landvik ....................................... 274
Chemiluminescence-Based Method for Genotyping Tg.AC
Responder Mice
D.C. Kantz, G.D. Lacks and R.E. Cannon ...................... 278
Accurate mRNA Size Determination in Northern Analysis Using
Individual Lane Size Markers
J.J. Doran, J.M. Sands and R.T. Timmer 280
Magnetic Separation to Concentrate the Estrogen Receptor from
Adipose Tissue for Western Analysis
B.H. Welter and T.M. Price ............................. 282
Simple Mixing Device to Reproducibly Prepare Cationic
Lipid-DNA Complexes (Lipoplexes)
S. Hirota, C. Tros de llarduya, L.G. Barron and F.C. Szoka, Jr. 286
CO2-Enriched Atmosphere on the Microscope Stage
G. Diaz, R. Isola, A.M. Falchi and A. Diana 292
Polyacrylamide Gel Fixation by Dryng and Gluing to Both Paper
and Transparent Plastic
Y.V. Guervaziev 294
Internet On-Ramp
A Virtual Molecular Biology Teaching Laboratory
R.M. Horton and R.C. Tait ...... ........................ . 298
Short Technical Reports
Screening ofTransgenic Plants by Amplification of Unknown
Genomic DNA Flanking T-DNA
D. Spertini, C. Beliveau and G. Bellemare 308
Nonradioactive Labeling of Large DNA Fragments for Genome
Walking, RFLP and Northern Blot Analysis
R Adhami, S. MUllerandM.-T. Hauser ....................... 314
Differentiation ofHard-to-Type Bacterial Strains by RNA
Mismatch Cleavage
B.J. Bricker ....................................... 321
Simplified Gene-Fragment Phage Display System for
Epitope Mapping
S. Gupta, K. Arora, A. Sampath, S. Khurana, S.S. Singh, A. Gupta
and V.K. Chaudhary ................................. . 328
High-Throughput DNA Extraction from Old Paraffin-
Embedded Biopsies
J. Jalouli, L. Sand, B. Gustavsson, J.M. Hirsch and R-A. Larsson 334
BioFeedback .................................... 340
Research Reports
Seven-Color, Homogeneous Detection of Six PCR Products
L.G. Lee, K.J. Livak, B. Mullah, R.J. Graham, R.S. Vinayak
and T.M. Woudenberg ................................ 342
Direct Observation ofNucleocytoplasmic Transport by Microinjection
ofGFP-Tagged Proteins in Living Cells
O. Rosorius, P. Heger, G. Stelz, N. Hirschmann, J. Hauber and R.H. Stauber 350
Flow Microsphere Immunoassay-Based Method of Virus Quantitation
T.I. Sarnoylova and B.F. Smith ............................ . 356
Laser Microdissection and Laser Pressure Catapulting for the
Generation of Chromosome-Specific Paint Probes
L. Schermelleh, S. Thalhammer, W. Heckl, H. Posi, T. Cremer,
K. Schtitze and M. Cremer .............................. 362
Strep-tag® II for One-Step Affinity Purification of Active bHLHzip
Domain of Human c-Myc
N. Zwicker, K. Adelhelm, R. Thiericke, S. Grabley and F. Hanel .......... 368
New Products ................................... 376
Index to Advertisers ............................. . 383
_______________________________________
BIOTECHNIQUES
VOL. 27, NO. 3, SEPTEMBER 1999
Benchmarks
Sequencing of Genomic DNA
U. Mahiknecht, D. Hoelzerand R. Bucala 406
Generation of Full-Length cDNA Library from Single Human
Prostate Cancer Cells
S.-Y. Ying, H.M. Lui, S.-L. Lin andC.-M. Chuong 410
Multistep-Touchdown Vectorette-PCRA Rapid Technique for the
Identification ofIVS in Genes
C. Rlibie, E. SchuIze-Bahr, M. Borggrefe, W. Haverkamp and G. Breithardt 414
Rapid, Sensitive Detection ofMutant Alleles in Codon 12 ofK-ras
by REMS-PCR
N.J. Roberts, H.L. Impey, T.L. Applegate, C.J. Fuery, R.L. Ward and A.V. Todd 418
Male-Specific Competitive PCR for the Quantification of Transplanted
Cells in Mice
N.J. Caron, P. Chapdelaine and J.P. Tremblay .................... 424
Construction of a Vector for Coupled In Vitro
Transcription/Translation
O. Ostersetzer and Z. Adam 428
Conditions for Permeabilization of Cells Used for Intracellular
Tyrosine Phosphorylation Studies
V. Chitu, D. Demydenko, G.K. Toth, Z. Hegedlis and E. Monostori 435
Improved Detection Reliability of P-Galactosidase in Histological
Preparations
G. Cheng, R.P. Thompson and R.G. Gourdie 438
Nucleic Acid Removal from Taq Polymerase Preparations Using an
Aqueous/Organic Biphasic System
A. Louwrier 444
Adaptation ofBTI-TNSBI-4 (High Five) Insect Cells for Large-Scale
Production in a Stirred Bioreactor
A.-C. Savary, B. Georges and C. Auriault 448
Single-Nucleotide Resolution of RNA Strands in the Presence of
their RNA Complements
J.J. Arnold, S.K.B. Ghosh, RC. Bevilacqua and C.E. Cameron 450
Improved Isolation of Nucleic Acids from Basidiomycete Fungi
P. Baldrian, J. Gabriel and M. Pospisek 458
Plasmid Harboring lac Represser and tTA Activator Genes Can
Regulate Two Inducible Genes in Mammalian Cells
T.-Y. Chang, Y.-Y. Wen, H.-H. Yeh, S.-T. Wang, I.-J. Su and H.-S. Liu 466
Internet On-Ramp
An Introduction to Peri: the QuizMaker Revisited
R.M. Horton 470
Short Technical Reports
Construction of Larger-Size Sequencing Templates from
Degraded DNA
I. Nasidze and M. Stoneking . 480
Recovery of Upstream cDNA Sequences by a PCR-Based Biotin-
Capture Method
S. Graslund, M. Larsson, F. Sterky, M. Uhlen, J. Lundeberg, C. Hoog
and S. Stahl 488
Universal Linker and Ligation Procedures for Construction of
Genomic DNA Libraries Enriched for Microsatcilites
M.B. Hamilton, E.L. Pincus, A. Di Fiore and R.C. Fleischer 500
Template Secondary Structure Promotes Polymerase Jumping
During PCR Amplification
V.K. Viswanathan, K. Krcmarik and N.P. Cianciotto 508
Competitive RT-PCR for Measuring Metalloprotcinase Gene
Expression in Human Mesangial Cells Exposed to a Hyperglycemic
Environment
A.K. Death, D.K. Yue and J.R. Turtle 512
Application of In Situ PCR to Yeast Cells for Screening YAC
Libraries
S. Aytay, L.M. Davis and J.C. Hozier 520
Research Reports
Design Strategies and Performance of Custom DNA
Sequencing Primers
G.A. Buck, J.W. Fox, M. Gunthorpe, K.M. Hager, C.W. Naeve,
R.T. Pon, P.S. Adams and J. Rush 528
High-Throughput Single Nucleotide Polymorphism Genotyping
by Fluorescent 5' Exonuclease Assay
P.A. Morin, R. Saiz and A. Monjazeb 538
Differential Display Probes for cDNA Arrays
T Trenkle, J. Welsh and M. McClelland 554
Quantitative RT-PCR to Evaluate In Vivo Expression of Multiple
TYansgenes Using a Common Intron
J. Fairman, L. Roche, 1. Pieslak, M. Lay, S. Corson, E. Fox, C. Luong,
G. Koe, B. Lemos, R. Grove, L. Fradkin and J. Vernachio 566
Introduction of an N-Glycosylation Cassette into Proteins at Random
Sites: Expression ofNeoglycosylated FGF
A. Yoneda, M. Asada, M. Suzuki and T. lrnarnura 576
Fabrication ofMicroarray of Gel-Immobilized Compounds on a
Chip by Copolymerization
A.V. Vasiliskov, E.N. Tirnofeev, S.A. Surzhikov, A.L. Drobyshev,
V.V. Shick and A.D. Mirzabekov 592
Product Application Focus
Tyramide Signal Amplification (TSA)-FISH Applied to Mapping
PCR-Labeled Probes Less Than I kb in Size
L.M. Schrimi, H.M. Padilla-Nash, A. Coleman, P. Moen, W.G. Nash,
J. Menninger, G. Jones, T. Ried and M. Dean 608
New Products 614
Index to Advertisers 623