BIOTECHNOLOGY PROGRESS

MARCH/APRIL 1999 VOLUME 15, NUMBER 2

TOPICAL PAPER__________________________________________

147 Application of Enzymes in the Pulp and Paper Industry

Pratima Bajpai

APPLIED CELLULAR PHYSIOLOGY AND METABOLIC ENGINEERING_______________________________________

158 Expression of Vitreoscilla Hemoglobin in Escherichia coli

Enhances Ribosome and tRNA Levels: A Flow Field-Flow Fractionation Study Mikael Nilsson, Pauli T. Kallio, James E. Bailey, Leif Biilow, and Karl-Gustav Wahiund*

164 Secretory Production of Recombinant Protein by a High Cell Density Culture of a Protease Negative Mutant Escherichia coli Strain

Si Jae Park, George Georgiou, and Sang Yup Lee*

168 Use of Mannosamine for Inducing the Addition of Outer Arm

N-Acetylglucosamine onto N-Linked Oligosaccharides of Recombinant Proteins in Insect Cells

Michael Donaldson, H. Alan Wood, Peter C. Kulakosky, and Michael L. Shuler*

BIOCATALYSTS AND BIOREACTOR DESIGN_____________

174 Application of a Fed-Batch System To Produce RNA by In Vitro

Transcription Jeffrey A. Kern and Robert H. Davis*

185 Performance Trade-offs in In Situ Chemostat NMR C. D. Castro, A. P. Koretsky, and M. M. Domach*

196 Modification of the Carbohydrate Composition of Sulfite Pulp by Purified and Characterized beta-Xylanase and beta-Xylosidase of Aureobasidium pullulans

L. P. Christov,* J. Myburgh, F. H. O'Neill, A. Van Tonder, and B. A. Prior

201 Metabolic Pathway of Propionibacterium Growing with Oxygen: Enzymes, 13C NMR Analysis, and Its Application for Vitamin Bi2 Production with Periodic Fermentation Kaiming Ye, Miyako Shijo, Kenichiro Miyano, and Kazuyuki Shimizu*

208 Hydrazide-Functionalized Poly(2-hydroxyethyl methacrylate) Microspheres for Immobilization of Horseradish Peroxidase Daniel Horak,* Michal Karpisek, Jaroslava Turkova, and Milan Benes

____________________________________________________________________________________________________________________________________

BIOSEPARATIONS AND DOWNSTREAM PROCESSING

216 Potential of Enzymatic Kinetic Resolution Using Solid Substrates Suspension: Improved Yield,

Productivity, Substrate Concentration, and Recovery A. Wolff, V. van Asperen, A. J. J. Straathof,* and J. J. Heijnen

228 Biosorption of Lead, Cadmium, and Zinc by Citrobacter Strain MCM B-181: Characterization Studies

P. R. Puranik and K. M. Paknikar*

238 Capture of Rare Cells in Suspension with Antibody-Coated Polystyrene Beads Shawn M. Gornez, Garry Choy, Norman Kabir, and Edward F. Leonard*

CELL CULTURE AND TISSUE ENGINEERING____________

245 Measurement of Bacterial Flagellar Thrust by Negative Dielectrophoresis

Michael P. Hughes and Hywel Morgan*

FORMULATION AND ENGINEERING OF BIOMATERIALS

250 Binding of Water, Oil, and Bile Acids to Dietary Fibers of the Cellan Type

Gerhard Dongowski* and Rudolf Ehwald

PROCESS SENSING AND CONTROL______________________

259 Modeling the Response Time of an In Vivo Glucose Affinity Sensor Haley R. Clark, Timothy A. Barbari,* and Govind Rao

NOTES________________________________________________________

267 Development of a Cell-Loaded Biosupport Separator for Continuous Immobilized-Cell Perfusion Culture

Tae Ho Lee, Gie-Taek Chun, and Yong Keun Chang*

273 Single, Combined, or Sequential Action of Pressure and Temperature on Lipoxygenase in Green Beans

{Phaseolus vulgaris L.): A Kinetic Inactivation Study Indrawati, Ann M. Van Loey, Linda R. Ludikhuyze, and Marc E. Hendrickx*

278 Direct Agrobacterium tumefaciens-Medmted Transformation otHyoscyamus muticus Hairy Roots Using

Green Fluorescent Protein Colleen D. Merritt, Surabhi Raina, Nina Fedoroff, and Wayne R. Curtis*

283 Purification of Human lnterleukin-2 Fusion Protein Produced in Insect Larvae Is Facilitated by Fusion with Green Fluorescent Protein and Metal Affinity Ligand Hyung Joon Cha, Nimish G. Dalal, Minh-Quan Pham, and William E. Bentley*

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BIOTECHNOLOGY PROGRESS
VOLUME 15, NUMBER 3, MAY/JUNE 1999



EDITORIAL

Functional Genomics: An Opportunity for Bioengineers 287
Michael L. Shuler



FUNCTIONAL GENOMICS: AN ENGINEERING
PERSPECTIVE
Toward Metabolic Phenomics: Analysis of Genomic Data Using 288
Flux Balances
Christophe H. Schilling, Jeremy S. Edwards, and
Bernhard 0. Palsson*


Metabolic Pathway Analysis: Basic Concepts and Scientific 296
Applications in the Post-genomic Era
Christophe H. Schilling, Stefan Schuster,
Bernhard 0. Palsson,* and Reinhart Heinrich




Functional Genomic Technologies: Creating New Paradigms for 304
Fundamental and Applied Biology
Camilla M. Kao



Proteomics: Theoretical and Experimental Considerations 312
Vassily Hatzimanikatis, Leila H. Choe, and Kelvin H. Lee*



TOPICAL PAPER
Design of Mixing Systems for Plant Cell Suspensions in Stirred 319
Reactors
Pauline M. Doran*



BIOCATALYSTS AND BIOREACTOR DESIGN
Stable Production of a Human Growth Hormone Antagonist from 336
CHO Cells Adapted to Serum-Free Suspension Culture
Raj Haldankar,* John J* Kopchick, and Darin Ridgway



High-Rate Treatment of Terephthalate in Anaerobic Hybrid 347
Reactors
Robbert Kleerebezem,* Maarit lvalo, Look W. Hulshoff Pol, and
Gatze Lettinga



Biotransformation and Biodegradation of Selected Nitroaromatics 358
under Anaerobic Conditions
Elias Razo-Flores,* Gatze Lettinga, and Jim A. Field


Immobilization of Highly Concentrated Cell Suspensions Using 366
the Laminar Jet Breakup Technique
Harry R. Brandenberger* and Fritz Widmer



Model Simulation and Analysis of Perfusion Culture of 373
Mammalian Cells at High Cell Density
A.-P. Zeng* and W.-D. Deckwer




Construction of a Thread Coaler and Use of Azocasein Release To Characterize the Sealant Coat Porosity 383
of Fibers Coated with Latex Biocatalytic Coatings
Michael C. Flickinger,* Ashim Mullick, and David F. Oilis



APPLIED CELLULAR PHYSiOLOGY AND METABOLIC
ENGINEERING

Constrained Cell Recognition Peptides Engineered into Streptavidin 391
Todd C. McDeviU, KJOII E. Nelson, and Patrick S. Stayton*


Differential Inhibition of Retrovirus Transduction by Proteoglycans and Free Glycosaminoglycans 397
Joseph M. Le Doux, Jeffrey R. Morgan, and Martin L. Yarmush*


Metabolic Engineering from a Cybernetic Perspective. 1. Theoretical Preliminaries 407
J. Varner and D. Rarnkrishna*


Metabolic Engineering from a Cybernetic Perspective. 2. Qualitative Investigation of Nodal 426
Architechtures and Their Response to Genetic Perturbation
J. Varner and D. Rarnkrishna*


Effect of SecB Chaperone on Production of Periplasmic Penicillin Acylase m Escherichia coli 439
C. Perry Chou,* Jen-Hung Tseng, Bao-Yuan Kuo, Ko-Ming Lai, Ming-l Lin, and Hua-Kuo Lin



Effect of the Nitrogen Source on Pyruvate Content and Rheological Properties of Xanthan 446
J.-L. Flores Candia and W.-D. Deckwer*



Enhanced Conversion Rate of L-Phenylalanine by Coupling Reactions of Aminotransferases and 453
Phosphoenolpyruvate Carboxykinase in Escherichia coli K-12
Yun-Peng Chao,* Zhang Jian Lai, Ping Chen, and Jong-Tzer Chern



Stable High-Copy-Number Integration ofAspergillus oryzae a-AMYLASE cDNA in an Industrial Baker's 459
Yeast Strain
Almudena Nieto, Jose A. Prieto, and Pascual Sanz*



Functional Expression of Horseradish Peroxidase in E. coli by Directed Evolution 467
Zhanglin Lin, Todd Thorsen, and Frances H. Arnold*


BIOSEPARATIONS AND DOWNSTREAM PROCESSING
Effects of Added Yeast on Protein Transmission and Flux in Cross-Flow Membrane Microfiltration 472
Vinod T. Kuberkar and Robert H. Davis*


Oxidative Refolding of Denatured/Reduced Lysozyme Utilizing the Chaperone-like Function of Liposomes 480
and Immobilized Liposome Chromatography
Makoto Yoshimoto and Ryoichi Kuboi*


Strategies for Recombinant Protein Recovery from Canolaby Precipitation 488
Ferhana Zaman, Ann R. Kusnadi, and Charles E. Glatz*


Aqueous Two-Phase Systems Containing Urea: Influence on Phase Separation and Stabilization of 493
Protein Conformation by Phase Components
Christian Ramsch, Lutz B. Kleinelanghorst, Esther A. Knieps, Jörg Thommes, and Maria-Regina Kula*


Protein Adsorption onto Tentacle Cation-Exchange Hollow-Fiber Membranes 500
Silvia A. Camperi, Agustin A. Navarro del Caftizo, Federico J. Wolman, Eduardo E. Smolko,
Osvaldo Cascone,* and Mariano Grasselli



Protein Separation Using Affinity-Based Reversed Micelles 506
Yan Sun,* Lei Gu, Xiao-Dong Tong, Shu Bai, Sosaku Ichikawa, and Shintaro Furusaki


Purification of Recombinant Proteins Based on the Interaction between a Phenothiazine-Derivatized 513
Column and a Calixiodulin Fusion Tail
Vesna Schauer-Vukasinovic and Sylvia Daunert*


PROCESS SENSING AND CONTROL

Organophosphorus Hydrolase-Based Assay for Organophosphate Pesticides 517
K. R. Rogers,* Y. Wang, A. Mulchandani,* P. Mulchandani, and W. Chen




Quartz Crystal Microbalance Measurement of Self-Assembled Micellar Tubules of the Amphiphilic Decyl 522
Ester of D-Tyrosine and Their Enzymatic Polymerization
Kenneth A. Marx,* Tiean Zhou, and Rupmoni Sarma



In Situ Monitoring of an Escherichia coli Fermentation Using a Diamond Composition ATR Probe and 529
Mid-infrared Spectroscopy
Denise L. Doak and Janice A. Phillips*



BIOENGINEERING

Use of In Vitro Data for Construction of a Physiologically Based Pharmacokinetic Model for Naphthalene 540
in Rats and Mice To Probe Species Differences
D. J. Quick and M. L. Shuler*


Control of a Nonsquare Drug Infusion System: A Simulation Study 556
Ramesh R. Rao, Johnnie W. Huang, B. Wayne Bequette,* Howard Kaufman, and Rob J. Roy


FORMULATION AND ENGINEERING OF BIOMATERIALS
Development of a Novel Methodology To Validate Optimal Sterilization Conditions for Maximizing the 565
Texture Quality of White Beans in Glass Jars
Isabel M. L. B. Avila,* Chantal Smout, Cristina L. M. Silva, and Marc Hendrickx


NOTES

Thermoreactive Displacers for Anion Exchange and Hydroxyapatite Displacement Chromatography 573
Ruth Freitag,* Sabine Vogt, and Michael Modler


DSC Confirmation That Vitrification Is Not Necessary for Stabilization of the Restriction Enzyme EcoRI 577
Dried with Saccharides
Maria P. Buera, Silvia Rossi, Silvia Moreno, and Jörge Chirife*



Recovery of Thuringiensin with Cetylpyridinium Chloride Using Micellar-Enhanced Ultrafiltration 580
Process
Yew-Min Tzeng,* Hung-Yieng Tsun, and Yaw-Nan Chang
There is no Supporting Information for this issue.
* In papers with more than one author, the asterisk indicates the name of the author to whom inquiries about
the paper should be addressed.



_______________________________________


BIOTECHNOLOGY PROGRESS

VOLUME 15, NUMBER 4, JULY/AUGUST 1999


APPLIED CELLULAR PHYSIOLOGY AND METABOLIC
ENGINEERING

587 Effect of Polyphosphate Metabolism on the Escherichia coli
Phosphate-Starvation Response
Stephen J. Van Dien and J. D. Keasling*

594 Production of Acetone Butanol Ethanol (ABE) by a
Hyper-Producing Mutant Strain of Clostridium beijerinckii
BAIOI and Recovery by Pervaporation
Nasibuddin Qureshi and Hans P. Blaschek*

603 Production of D-p-HydroxyphenyIglycine by A^Carbarnoyl-D-amino
Acid Amidohydrolase-Overproducing Escherichia coli Strains
Yun-Peng Chao,* Tzong-Yuan Juang, Jong-Tzer Chern, and
Cheng-Kang Lee

PROCESS SENSING AND CONTROL

608 Adaptive Control at Low Glucose Concentration of HEK-293 Cell
Serum-Free Cultures
Pascal Siegwart, Johanne Cote, Keith Male, John H. T. Luong,
Michel Perrier, and Amine Kamen*

617 Predicting Fermentability of Wood Hydrolyzates with Responses
from Electronic Noses
Carl-Fredrik Mandenius,* Helena Liden, Tomas Ekiov,
Mohammad J. Taherzadeh, and Gunnar Liden

622 Determination of a Biological Absorber Depth Utilizing Multiple
Source—Detector Separations and Multiple Frequency Values of
Near-Infrared Time-Resolved Spectroscopy
Mark W. Hemelt and Kyung A. Kang*

630 Development and Validation of a Methodology for Intracellular
pH Measurements of Hybridoma Cells under Bioreactor Culture
Conditions
Marc Cherlet,* Patricia Franck, Pierre Nabet, and Annie Marc

BIOCATALYSTS AND BIOREACTOR DESIGN

640 Production ofa-Amylase in Fed-Batch Cultures of ugb+ and ugb-
Recombinant Escherichia coli: Some Observations
Nader Enayati, Canan Tari, Satish J. Parulekar,*
Benjamin C. Stark, and Dale A. Webster

646 Long-Term Mechanical and Biological Stability of an Immobilized
Cell Reactor for Continuous Mixed-Strain Mesophilic Lactic
Starter Production in Whey Permeate
L. Lamboley, C. Lacroix,* J. M. Artignan, C. P. Champagne, and
J. C. Vuillemard


655 The Production of Monoclonal Antibody in Growth-Arrested Hybridomas Cultivated in Suspension and
Immobilized Modes
Douglas B. Seifert and Janice A. Phillips*


667 The Incidence of Oscillatory Behavior in the Continuous Fermentation of Zymomonas mobilis
P. James McLellan,* Andrew J. Daugulis, and Jinghong Li


681 Kinetics of the Bioleaching of Chalcopyrite Concentrate by Acidophilic Thermophile Acidianus brierleyi
Yasuhiro Konishi,* Satoru Asai, Masahiko Tokushige, and Torn Suzuki '

689 Conformationally Changed Cytochrome c-Mediated Fusion of Enzyme- and Substrate-Containing
Liposomes
Makoto Yoshimoto, Peter Walde, Hiroshi Umakoshi, and Ryoichi Kuboi*

697 Determination of the Reaction Yield during Biotransformation of the Volatile and Chemically Unstable
Compound B-lonone by Aspergillus niger
Franck Grivel, Christian Larroche,* and Jean Bernard Gros

706 Bioconversion of Recalcitrant 4-Methyldibenzothiophene to Water-Extractable Products Using
Lignin-Degrading Basidiomycete Coriolus versicolor
Hirofumi Ichinose, Hiroyuki Wariishi,* and Hiroo Tanaka

BIOENGINEERING

715 Tissue Engineering Human Placenta Trophoblast Cells in 3-D Fibrous Matrix: Spatial Effects on Cell
Proliferation and Function
Teng Ma, Yan Li, Shang-Tian Yang,* and Douglas A. Kniss*

BIOSEPARATIONS AND DOWNSTREAM PROCESSING

725 Development of Process Flow Sheets for the Purification of Supercoiled Plasmids for Gene Therapy
Applications
Guilherme N. M. Ferreira, Joaquim M. S. Cabral, and Duarte M. F. Prazeres* '

732 Influence of Protein Surface Morphology on the Ultrafiltration Flux Resistance of BovilTe Serum Albumin
Belinda Elysee-Collen and Robert W. Lencki*

740 Kinetic Study of the Concentration Dependence of the Mass Transfer Rate Coefficient in Anion-Exchange
Chromatography of Bovine Serum Albumin
Kanji Miyabe and Georges Guiochon* /

753 Electrophysiological Model of Intact and Processed Plant Tissues: Cell Disintegration Criteria
Alexander Angersbach, Volker Heinz, and Dietrich Knorr*

FORMULATION AND ENGINEERING OF BIOMATERIALS

763 Production of Microspheres with Surface Amino Groups from Blends of Poly(Lactide-co-glycolide) and
Poly(E-CBZ-L-lysine) and Use for Encapsulation
Ji Zheng and Peter J. Hornsby*

NOTES

768 Electrostatic Encapsulation and Growth of Plant Cell Cultures in Alginate
Hamad A. AI-Hajry, Salha A. AI-Maskry, Latifa M. AI-Kharousi, Osman EI-Mardi, Walid H. Shayya, and
Mattheus F. A. Goosen*