EXPERIMENTAL PARASITOLOGY

Volume 91, Number I, January 1999

CONTENTS

Regular Articles

Gabriele Wildenburg and Kimberly Henkle-Duhrsen. Onchocerca volvulus: Immunolocaliza-
tion of the Extracellular CuZn Superoxide Dismutase Using Antibodies Raised against a
15-mer Epitope of This Enzyme .............................................................................................................1

Claudia N. Paiva, Morgana T. L. Castelo-Branco, Joseli Lannes-Vieira, and Cerii R. Gattass.
Trypanosoma cruzi: Protective Response of Vaccinated Mice Is Mediated by CD8^ Cells,
Prevents Signs ofPolyclonal T Lymphocyte Activation, and Allows Restoration of a Resting
Immune State after Challenge ............................................................................................................... 7

Helene Lejoly-Boisseau, Martine Appriou, Martine Seigneur, Annie Pruvost, Jeanne Tribouley-
Duret, and Jean Tribouley. Schistosoma mansoni: In Vitro Adhesion of Parasite Eggs to
the Vascular Endothelium. Subsequent Inhibition by a Monoclonal Antibody Directed to
a Carbohydrate Epitope ........................................................................................................................ 20

F. Migot-Nabias, B. Ollomo, G. Dubreuil, A. Morelli, 0. Domarle, R. Nabias, A. J. Georges,
and P. Millet. Plasmodium coatneyi: Differential Clinical and Immune Responses of Two
Populations of Macaca fascicularis from Different Origins....................................................................... 30

Roger W. Stich, Allison C. Rice-Ficht, Wenbin Tuo, and Wendy G. Brown. Babesia bovis:
Common Protein Fractions Recognized by Oligoclonal B. bovis-Specific CD4+ T Cell Lines
from Genetically Diverse Cattle ................................................................................................................. 40

Nancy A. Mercer, Jeffrey R. McKelvey, and Carmen F. Fioravanti. Hymenolepis diminuta:
Catalysis of Transmembrane Proton Translocation by Mitochondrial NADPH —> NAD
Transhydrogenase...................................................................................................................................... 52

Carl A. Lowenberger, Sofie Kamal, Jody Chiles, Susan Paskewitz, Philippe Bulet, Jules A.
Hoffmann, and Bruce M. Christensen. Mosquito-Plasmodium Interactions in Response
to Immune Activation of the Vector ............................................................................................................ 59

Carol L. Eisenberger and Charles L. Jaffe. Leishmania: Identification of Old World Species
Using a Permissively Primed Intergenic Polymorphic-Polymerase Chain Reaction................................. 70

Harris L. Rotman, Thomas M. Daly, and Carole A. Long. Plasmodium: Immunization with
Carboxyl-Terminal Regions ofMSP-l Protects against Homologous but Not Heterologous
Blood-Stage Parasite Challenge................................................................................................................... 78

Gary W. Gervais, Kathy Trujillo, Brian L. Robinson, Wallace Peters, and Adelfa E. Serrano.
Plasmodium berghei: Identification of an mdr-\ike Gene Associated with Drug Resistance ................. 86

Research Briefs

Adelfa E. Serrano, Brian L. Robinson, Wallace Peters, and Kathy Trujillo-Nevarez. Plasmodium
berghei and Plasmodium yoelii: Molecular Karyotypes of Drug-Resistant Lines....................................... 93

EXPERIMENTAL PARASITOLOGY

Volume 91, Number 2, February 1999

Mark Loyevsky, John B. Sacci, Jr., Patricia Boehme, William Weglicki, Christy John, and
Victor R. Gordeuk. Plasmodium falciparum and Plasmodium yoelii: Effect of the Iron
Chelation Prodrug Dexrazoxane on in Vitro Cultures........................................................... 105

William Trager, Gokal S. Gill, Christine Lawrence, and Ronald L. Nagel. Plasmodium falci
parum: Enhanced Gametocyte Formation in Vitro in Reticulocyte-Rich Blood................... 115

Corinne Capet, Nathalie Kapel, Jean Francois Huneau, Denis Magne, Rene Laikuen, Viviane
Tricottet, Yves Benhamou, Daniel Tome, Jean Gerard Gobert. Cryptosporidium paruum
Infection in Suckling Rats: Impairment of Mucosal Permeability and Na +-Glucose
Cotransport........................................................................................................................ 119

Kerrie Tosh, Sandie Cheesman, Paul Horrocks, and Brian Kilbey. Plasmodium falciparum:
Stage-Related Expression of Topoisomerase 1........................................................................ 126

Antonio Barragan, Dorothe Spillmann, Peter G. Krernsner, Mats Wahigren, and Johan Carlson.
Plasmodium falciparum: Molecular Background to Strain-Specific Rosette Disruption by
Glycosaminoglycans and Sulfated Glycoconjugates ................................................................ 133

Ayrnan S. Hussein, Michael E. Grigg, and Murray E. Selkirk. Nippostp'ongylus brasiliensis:
Characterisation of a Somatic Amphiphilic Acetylcholinesterase with Properties Distinct
from the Secreted Enzymes ..................................................................................................... 144

Sabina Belli, Andrea Formenton, Tanja Noll, Alasdair lvens, Raymond Jacquet, Chantal
Desponds, Denise Hofer, and Nicolas Fasel. Leishmania major: Histone HI Gene Expres-
sion from the siv3 Locus.........................................................................................................151

K. P. Piper, D. J. Roberts, and K. P. Day. Plasmodium falciparum: Analysis of the Antibody
Specif icily to the Surface of the Trophozoite-infected Erythrocyte ...................................... 161

Cristina Paveto, Hector N. Torres, Mirtha M. Flawia, Matilde Garcia-Espitia, Arturo Ortega,
and Esther Orozco. Entamoeba histolytica: Signaling through G Proteins ......................... 170

Martha C. Mutomba, Fengsheng Li, Keith M. Gottesdiener, and Ching C. Wang. A Trypanosoma
brucei Bloodstream Form Mutant Deficient in Ornithine Decarboxylase Can Protect
against Wild-Type Infection in Mice........................................................................................ 176

Julia W. Albright, Ronnie C. Mease, Carol Lambert, and Joseph F. Albright. Trypanosoma
musculi: Tracking Parasites and Circulating Lymphoid Cells in Host Mice ....................... 185

Research Briefs

Jean-Marc Derothe, Nathalie Le Brun, Claude Loubes, Marco Perriat-Sanguinet, and Cather
ine Moulia. Trypanosoma musculi: Compared Levels ofParasitosis in Wild and Laboratory
Strains of Mus musculus Mice................................................................................................. 196

Maarten Hoek, Hui Xu, and George A. M. Cross. Trypanosoma brucei: Generation of Specific
Antisera to Recombinant Variant Surface Glycoproteins...................................................... 199
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EXPERIMENTAL PARASITOLOGY

Volume 91, Number 3, March 1999

D. J. P. Ferguson, M-F. Cesbron-Delauw, J-F. Dubremetz, L. D. Sibley, K. A. Joiner, and
S. Wright. The Expression and Distribution of Dense Granule Proteins in the Enteric
(Coccidian) Forms of Toxoplasma gondii in the Small Intestine of the Cat ........................ 203

Young-Ha Lee, Jacqueline Y. Channon, Tadashi Matsuura, Joseph D. Schwartzman, Dae-
Whan Shin, and Lloyd H. Kasper. Functional and Quantitative Analysis of Splenic T Cell
Immune Responses Following Oral Toxoplasma gondii Infection in Mice........................... 212

Keith Wilson, Lyie Uyetake, and John Boothroyd. Trypanosoma brucei: Cis-Acting Sequences
Involved in the Developmental Regulation of PARP Expression.......................................... 222

Jerome Ricard, Herve Pelloux, Anne-Laure Favier, Uwe Gross, Elisabeth Brambilla, and Pierre
Ambroise-Thomas. Toxoplasma gondii: Role of the Phosphatidylcholine-Specific Phospholi-
pase C during Cell Invasion and Intracellular Development................................................ 231

John W. Barnwell, Mary R. Galinski, S. Giovanni DeSimone, Francine Perler, and Paul Ingra-
vallo. Plasmodium uiuax, P. cynomolgi, and P. knowlesi: Identification ofHomologue Pro-
teins Associated with the Surface of Merozoites.................................................................... 238

Nicholas C. Sangster, Sharron C. Bannan, Anthony S. Weiss, Susan C. Nulf, Ronald D. Klein,
and Timothy G. Geary. Haemonchus contortus: Sequence Heterogeneity ofinternucleotide
Binding Domains from P-Glycoproteins and an Association with Avermectin/Milbemycin
Resistance.................................................................................................................................. 250

Jurgen F. J. Kun, Karena L. Waller, and Ross L. Coppel. Plasmodium falciparum: Structural
and Functional Domains of the Mature-Parasite-Infected Erythrocyte Surface Antigen.................. 258

Myrna G. Serrano, Luiz R. Nunes, Marta Campaner, Gregory A. Buck, Erney P. Camargo,
and Marta M. G. Teixeira. Trypanosomatidae: Phytomonas Detection in Plants and Phy-
tophagous Insects by PCR Amplification of a Genus-Specific Sequence of the Spliced Leader
Gene........................................................................................................................................... 268

Research Briefs

Andreas Kampkotter, lan Ridgers, David A. Johnston, David Rollinson, Werner Kunz, and
Christoph G. Grevelding. Schistosoma mansoni: Cloning and Characterization of the Ras
Homologue................................................................................................................................. 280

Martin F. Heyworth, Jason D. Foell, Thomas W. Sell. Giardia muris: Evidence for a (beta)-Giardin
Homologue................................................................................................................................. 284

EXPERIMENTAL PARASITOLOGY
Volume: 92, Number:1, May 1999

George R. Gibson, David Ramirez, Julie Maier, Cynthia Castillo, and Siddhartha Das. Giardia
lamblia: Incorporation of Free and Conjugated Fatty Acids into Glycerol-Based
Phospholipids............................................................................................................................ I

P. V. Lalitha, Pawan Malhotra, Rana Chattopadhyay, and V. S. Chauhan. Plasmodium faici-
parum: Variations in the C-Terminal Cysteine-Rich Region of the Merozoite Surface Pro-
tein-1 in Field Samples among Indian Isolates...................................................................... 12

G. Dreyfuss, P. Vignoles, D. Rondelaud, and C. VareiUe-Morel. Fasciola hepatica: Characteris-
tics of Infection in Lymnaea truncatula in Relation to the Number ofMiracidia at Exposure 19

Michael K. Shaw. Theileria parva: Sporozoite Entry into Bovine Lymphocytes Is Not Dependent
on the Parasite Cytoskeleton................................................................................................... 24

J.-P. Paiement, R. K. Prichard) and P. Riheiro. Haemonchus contortus: Characterization of a
Glutamate Binding Site in Unselected and lvermectin-Selected Larvae and Adults......... 32

Jindrich Soltys, Prem K. Goyal, and Derek Wakelin. Cellular Immune Responses in Mice
Infected with the Intestinal Nematode Trichuris muris........................................................ 40

Stuart J. Kahn, Don Nguyen, Jason Norsen, Monika Wieklinski, Tracy Granston, and Maria
Kahn. Trypanosoma cruzi: Monoclonal Antibodies to the Surface Glycoprotein Superfamily
Differentiate Subsets of the 85-kDa Surface Glycoproteins and Confirm Simultaneous
Expression of Variant 85-kDa Surface Glycoproteins............................................................ 48

Joaquina Martin-Sanchez and Pedro Garcia-Fernandez. Theileria annulata: Genetic Charac-
terization of Spanish Isolates by Isoenzyme Electrophoresis and Random Amplified Poly-
morphic DNA............................................................................................................................. 57

Alain Ferrer, Johan Hoebeke, and Daniel Bout. Purification and Characterization of Two a-
Amylases from Toxoplasma gondii.......................................................................................... 64

Luis Favila-Castillo, Amalia Monroy-Ostria, and David Garcia-Tapia. Plasmodium chabaudi
chabaudi: Effect of Low Parasitemias on Immunity in CB6FI Mice ................................... 73

Bert Mulder, Ton Lensen, Timoleon Tchuinkarn, Will Roeffen, Jan Peter Verhave, Christian
Boudin, and Robert Sauerwein. Plasmodium falciparum: Membrane Feeding Assays and
Competition ELISAs for the Measurement of Transmission Reduction in Sera from
Cameroon................................................................................................................................... 81

EXPERIMENTAL PARASITOLOGY
Volume: 92, Number: 2, June 1999

Dana G. Mordue, Sebastian Hakansson, Ingrid Niesman, and L. David Sibley. Toxoplasma
gondii Resides in a Vacuole That Avoids Fusion with Host Cell Endocytic and Exocytic
Vesicular Trafficking Pathways ............................................................................................... 87

M. S. Gonzalez, N. F. S. Nogueira, C. B. Mello, W. De Souza, G. A. Schaub, P. Azambuja, and
E. S. Garcia. Influence of Brain and Azadirachtin on Trypanosoma cruzi Development in
the Vector, Rhodnius proUxus.................................................................................................. 100

G. Schares, J. F. Dubremetz, J. P. Dubey, A. Barwald, A. Loyens, and F. J. Conraths. Neospora
caninum: Identification of 19-, 38-, and 40-kDa Surface Antigens and a 33-kDa Dense
Granule Antigen Using Monoclonal Antibodies ..................................................................... 109

R. G. Bell, L. Adams, S. Coleman, D. Negrao-Correa, and T. Kiel. Brugiapahangi: Quantitative
Analysis of Infection in Several Inbred Rat Strains.............................................................. 120

Radhey S. Kaushik, Jude E. Uzonna, John R. Gordon, and Henry Tabel. Innate Resistance to
Trypanosoma congolense Infections: Differential Production of Nitric Oxide by Macro-
phages from Susceptible BALB/c and Resistant C57BI/6 Mice............................................. 131

David Artis, Christopher S. Potten, Kathryn J. Else, Fred D. Finkelman, and Richard K.
Grencis. Trichuris muris: Host Intestinal Epithelial Cell Hyperproliferation during
Chronic Infection Is Regulated by Interferon-gama...................................................................... 144

Research Brief

Tony Triglia and Alan F. Cowman. Plasmodium falciparum: A Homologue of^-Aminobenzoic
Acid Synthetase ...............................................................................................-...................... 154

Erratum

Volume 91, Number 1 (1999), in Article ID expr. 1998.4355, "Leishmania: Identification of Old
World Species Using a Permissively Primed Intergenic Polymorphic-Polymerase Chain
Reaction," by Carol L. Eisenberger and Charles L. Jaffe.................................................... - 159