CELL BIOLOGY (Vol. 9) January 1999

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1 Versatility in signalling: multiple responses to EGF receptor activation during Drosophila oogenesis
Cheryl Van Buskirk and Trudi Schupbach

5 Out of the ER - outfitters, escorts and guides

Johannes M. Herrmann, Per Malkus and Randy Schekman

HEADLINES

8 mutant mouse sheds light on storage-pool deficiency; pole positioned by pombe pore protein;
Q: which came first? A: The Eg.; a fab result; going green with every developmental stage;
a talin of two ends; replication in the early embryo

REVIEWS

11 PUTTING E. COU ON A PEDESTAL: a unique system to study signal transduction
and the actin cytoskeleton
Danika L. Goosney, Myriam de Grado and B. Brett Finlay

15 WALTZING WITH WASP
Narayanaswamy Ramesh, Ines M. Anton, Narcisa Martinez-Quiles and Raif S. Geha

20 RESPONDING TO ATTRACTION: chemotaxis and chemotropism in DictyosteliLim and yeast
Robert A. Arkowitz
28 BI-CYCLING THE FURIN PATHWAY: from TGN localization to pathogen activation
and embryogenesis
Sean S. Molloy, Eric D. Anderson, Francois Jean and Gary Thomas

MEETING REPORT

37 100 years of Golgi complexities

Colin Jamora

BOOK REVIEWS

40 Calmodulin and Signal Transduction (edited by Linda J. Van Eldik and D. Martin Watterson),
Thomas Grundstrom

40 Helix-Loop-Helix Transcription Factors (3rd edn) (by Trevor D. Littlewood and Gerard 1. Evan), Richard Deed

41 The Molecular Basis of Medical Cell Biology (by Gerald M. Fuller and Dennis Shields), Graham A. W. Rook

36 Techniques update: A genetics-friendly GFP assay,
contributed by Stephen Cronin and Randolph Hampton

36 Technical Tips Online

Careers-perspective
39 Warren Williams, data analyst at British Telecom Syncordia Solutions

CELL BIOLOGY (Vol. 9) February 1999

43 GFP ILLUMINATES EVERYTHING
Andrew Matus

44 SOFT X-RAY MICROSCOPY
Chris lacobsen

48 FLUORESCENCE LIFETIME IMAGING MICROSCOPY: spatial resolution of biochemical processes
in the cell
Philippe 1. H. Bastiaens and Anthony Squire

52 DUAL-COLOUR IMAGING WITH GFP VARIANTS
jan Ellenberg, Jennifer Lippincott-Schwartz and John F. Presley

57 USING GFP IN FRET-BASED APPLICATIONS
Brian A. Pollok and Roger Heim

61 PHOTOBLEACHING GFP REVEALS PROTEIN DYNAMICS INSIDE LIVE CELLS
Jamie White and Ernst Stelzer

66 IMAGING LIVING CELLS AND TISSUES BY TWO-PHOTON EXCITATION MICROSCOPY
David W. Piston

70 NEAR-FIELD SCANNING OPTICAL MICROSCOPY IN CELL BIOLOGY
Aaron Lewis, Anna Radko, Nilly Ben Ami, Daniel Palanker and Klony Lieberman

73 LOOKING DEEPER INTO VERTEBRATE DEVELOPMENT
Russell E. Jacobs, Eric T. Ahrens, Thomas J. Meade and Scott E. Fraser

77 ATOMIC FORCE MICROSCOPY: a powerful tool to observe biomolecules at work
Andreas Engel, Yuri Lyubchenko and Daniel Muller

81 ELECTRON TOMOGRAPHY OF MOLECULES AND CELLS
Wolfgang Baumeister, Rudo Grimm and jochen Walz

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60,69 Technical Tips Online

CELL BIOLOGY (Vol. 9) March 1999

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87 comment
Looking at lipid rafts?
Ken )acobson and Christian Dietrich

92 letter

A future for electron microscopy in cell biology?
Hans j. Geuze

94 cilia whip up left-right asymmetry; no centriole, no centrosome; apoptosis and spindle-assembly united;
800 oscillations per cycle; clearing the air on an Aurora/lpl2 family member

96 FUNCTIONAL DIVERSITY IN THE DYNAMIN FAMILY

Alexander M. van der Bliek

102 SIGNAL TRANSDUCTION BY A PROTEASE CASCADE

Ellen K. LeMosy, Charles C. Hong and Carl Hashimoto

107 GETTIN' DOWN WITH UBIQUITIN: turning off cell-surface receptors, transporters and channels

Linda Hicke

1 1 3 THE ROLE OF COP1 IN REPRESSION OF ARABIDOPSIS PHOTOMORPHOGENIC DEVELOPMENT

Mark T. Osterlund, Lay-Hong Ang and Xing Wang Deng

Feature on special-interest subgroup meetings at the ASCB
1 18 Regulation of actin dynamics by ADF/cofilins m vivo and in vitro

James R. Bamburg

119 Tau protein in neurodegenerative disease

Gloria Lee

119 Are there multiple roles for the Ran GTPase?

Mary Dasso

1 19 -y-Tubulin: questions outstanding

Patricia G. Wilson
120 Gap junctions
Paul D. Lampe and Eric C. Beyer

120 Nuclear dynamics at mitosis
Michael Mancini and J. Nickerson

121 Proteoglycans and cell adhesion
Anne Woods

121 An Electronic Companion to Molecular Cell Biology (by Robert Van Buskirk and Udaya K. Liyange),

Shamshad Cockcroft

122 Cyclin Dependent Kinase (CDK) Inhibitors (edited by P. K. Vogt and S. 1. Reed), Andreas Trumpp

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1 12 Technical Tips Online
Careers perspective
123 Megan Davies, Head of the MRC Centre Cambridge, Hills Road, Cambridge, UK

CELL BIOLOGY (Vol. 9) April 1999

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125 comment

PTEN: a tumour suppressor that functions as a phospholipid phosphatase
Tomohiko Maehama and lack E. Dixon

129 gamma tubulin and the origin of two-ness; PERKing up the ER stress response; rafts ready for
turning on T cells; the Golgi bypassed; aggresomes - a novel way to die slowly; getting in is easy,
but how to get out?; serum theorem

REVIEWS
132 perspective

REGULATION OF PROTEIN BIOGENESIS AT THE ENDOPL^SMIC RETICULUM
MEMBRANE
Ramanujan S. Hegde and Vishwanath R. Lingappa

138 RGS PROTEINS: more than just GAPs for heterotrimeric G proteins

Luc De Vries and Marilyn Gist Farquhar

144 THE SUBAPICAL COMPARTMENT: a novel sorting centre?

Sven C.D. van IJzendoom and Dick Hoekstra

150 TARGETING VESICLES TO SPECIFIC SITES ON THE PLASMA MEMBRANE: the role of the
sec6/8 complex
Shu-Chan Hsu, Christopher D. Hazuka, Davide L. Foletti and Richard H. Scheller

154 High-resolution electron cryomicroscopy of macromolecular assemblies
Wah Chiu, Amy McCough, Michael B. Sherman and Michael F. Schmid

159 Archaea: from genomics to physiology and the origin of life

Ute C. Vothknecht and Debra L. Tumbula

1 64 At the Bench: A Laboratory Navigator (by Kathy Barker),

Caroline E. Shamu

128 Technical Tips Online

Pictures in cell biology

162 Autophagosomes in yeast, produced in collaboration with Yoshinori Ohsumi

Careers-perspective

163 Dianna DeVore, patent counsel at Bozicevic and Reed, Palo Alto, USA

trends in C£LL BIOLOGY (Vol. 9) April 1999

trends in CELL BIOLOGY (Vol. 9) JUNE 1999

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207 comment

All aboard the cyclin train: subcellular trafficking of cyclins and their CDK partners
Jing Yang and Sally Kornbluth

211 letter

PH domains in WASP - a bug in the system?
Robert Insall and Laura Machesky *

212 reply
Hiroaki Miki and Tadaomi Takenawa

212 letter

Landing on lipid rafts
Marc Fivaz, Laurence Abrami and F. Gisou van der Coot

HEADLINES
214 dinners are forever; centrosome birth control; PP2A joins the Wnt team; VASP moves forward with Listeria

216 AKAPs: from structure to function
Marcie Colledge and John D. Scott ^-oc^.

222 PROTEIN IMPORT INTO CHLOROPLASTS
Xuejun Chen and Danny J. Schnell )^^

228 CYTOSKELETAL REARRANGEMENT DURING MIGRATION AND ACTIVATION OF T LYMPHOCYTES

)uan M. Serrador, Marta Nieto and Francisco Sanchez-Madrid

233 HOMING AND MOBILIZATION IN THE STEM CELL NICHE

Anthony D. Whetton and Gerry \. Graham

239 Microbial fusion
Deborah Sweet

241 Proteolysis by presenilins and the renaissance of tau

Christian Haass and Eckhard Mandelkow

246 Antigen Presentation (Curr. Top. Microbiol. Immunol. 232) (edited by J. Lindsay Whitton et o/.),

Mar Fernandez-Borja

247 Biomedical Electron Microscopy. Illustrated Methods and Interpretations (by Arvid B. Maunsbach and

BjOrn A. Afzelius), Judith Klumperman

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227 Technical Tips Online

244 Cyto bytes

Careers-perspective

245 Norka Ruiz Bravo, Deputy Director of the Division of Cancer Biology at the National
Cancer Institute, USA

trends in CELLBIOLOGY

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249 comment
First the CDKs, now the DDKs
LeLand H. Johnston, Hisao Masai and Akio Sugino

253 update
Is dynamin a regular motor or a master regulator?
Alexander M. van der Bliek

HEADLINES

254 stathmin/0p18: catastrophe again?; DIY at the bench: plentiful PI 3-kinases complicate chemotaxis;
Tyro-3, Axl and Mer: sperm support group; getting your actin together with WASP; get your stacks
together!; proteasomes - an appetite for mRNA

REVIEWS
257 perspective
GERMLINE CYSTS: a conserved phase of germ cell development?
Melissa E. Pepling, Margaret de Cuevas and Allan C. Spradling

262 Hsp90's SECRETS UNFOLD: new insights from structural and functional studies
Avrom 1. Caplan

268 OCCLUDIN AND CUUJDINS IN TIGHT-JUNCTION STRANDS: leading or supporting players?
Shoichiro Tsukita and Mikio Furuse

274 REGULATION OF SMAD SIGNALLING BY PROTEIN ASSOCIATIONS AND
SIGNALLING CROSSTALK
Ying Zhang and Rik Derynck

PIONEERS

280 RICHARD W.Young: and the band marched on
Steven j. Fliesler and Dean Bok

TECHNIQUES
284 Use of caged fluorochromes to track macromolecular movement in living cells
loan C. Politz

BOOK REVIEW

289 Biological Sequence Analysis. Probabilistic Models of Proteins and Nucleic Acids
(by R. Durbin, S. Eddy, A. Krogh and G. Mitchison), Frank Eisenhaber
273 Technical Tips Online

Pictures in cell biology
283 Squaring up to the cell-shape debate
Christopher S. Chen, Clifford Brangwynne and Donald E. Ingber

Careers-perspective
288 Susan King, Executive Editor at John Wiley & Sons

289 Authors' correction

TRENDS IN CELL BIOLOGY
VOL. 9 , NO. 8 , AUGUST 1999

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291 comment
Glycans in post-Golgi apical targeting: sorting signals or structural props?
Enrique Rodriguez-Boulan and Alfonso Gonzalez

295 comment
Cryptochromes - bringing the blues to circadian rhythms
Paul F. Devlin and Steve A. Kay

HEADLINES
299 on a snailroad to the nucleus; Ran regulates spindle assembly; hitchhiking across membranes; a
Golgi portrait: new perspectives on old problems; connecting to the laminin network

REVIEWS
302 NUCLEAR BODIES: multifaceted subdomains of the interchromatin space
A. Gregory Matera

312 GETTING ACROSS THE NUCLEAR PORE COMPLEX
Bradford Talcott and Mary Shannon Moore
See also the 'Pictures in cell biology' feature on pp. 310-311

319 INTEGRIN-LINKED KINASE (ILK): a regulator of integrin and growth-factor signalling
Shoukat Dedhar, Benjamin Williams and Gregory Hannigan

323 AN EXEGESIS OF lAPs: salvation and surprises from BIR motifs
Lois K. Miller

MEETING REPORT
329 Telomeres and telomerase: at the end, it all comes together
Sara K. Evans, Alison A. Bertuch and Victoria Lundblad

BOOK REVIEWS

331 The Shoot Apical Meristem: Its Growth and Development (by R. F. Lyndon), Keiko U. Torii and
Steven E. dark

332 Ubiquitin and the Biology of the Cell (edited by jan-Michael Peters, J. Robin Harris and
Daniel Finley), Phoebe R. Johnson

X calendar/classified

301 Technical Tips Online
Pictures in cell biology

310 Structures of nuclear-transport components
Produced in collaboration with Yuh-Min Chook, Gino Cingolani, Elena Conti, Murray Stewart,
Ingrid Vetter and Alfred Wittinghofer

Careers-perspective
333 Sally A. Amero, Scientific Review Administrator at the NIH, MD, USA

_______________________________________

TRENDS IN CELL BIOLOGY
VOL. 9 , NO. 10 , 1999



FORUM
377 comment
Titin: a molecular control freak
John Trinick and Larissa Tskhovrebova

381 comment
Shuffling the deck: plant signalling plays a club
Bruce D. Kohorn

HEADLINES

384 blues news; breakthroughs on mitotic and meiotic chromosome cohesion; NLK TAKs onto the
Wnt signalling pathway; lovastatin inhibits protein degradation?; motorized meiosis

REVIEWS

387 SEPTINS:cytoskeletal polymers or signalling CTPases?
Christine M. Field and Douglas KeUogg

394 PROTEIN TRANSLOCATION IN APOPTOSIS
Alan G. Porter

402 BACTERIAL TYPE If PROTEIN EXPORT AND PILUS BIOGENESIS: more than just
homologies?
David Nunn

409 UNRAVELLING THE KINETOPLASTIDPARAFLAGELAR ROD
John A, Maga and Jonathan H. LeBowitz

PIONEERS

413 George E. Palade: charting the secretory pathway
David D, Sabatini

BOOK REVIEWS

419 Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins (edited by Andreas
Baxevanrs and B, F. Francis OueUette), Jon Sund Blandfort

420 Green Fluorescent Proteins (Methods in Cell Biology, Vol. 58) (edited by Kevin F- Sullivan and
Steve A, Kay), Jamie White

421 Journal review: Nature Cell Biology, Mark Marsh
Careers-perspective

417 Elizabeth DouvHle, Fund Manager at GeneChem Technologies Venture Capital Fund LP.„
Montreal, Canada

X calendar/classified

Pictures in cell biology
418 From basic cell biology to cancer screening
Anthony D. Mills and Lesley Morris

420 Technical Tips Online